Day: November 3, 2022

Hamilton-Miller, J. M. T.; Grey, Daphne published an article in 1975, the title of the article was Resistance to trimethoprim in klebsiellae isolated before its introduction.Reference of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate And the article contains the following content:

In vitro 6 of 12 strains of Klebsiella aerogenes or K. ozaenae were resistant to trimethoprim lactate (I lactate) [23256-42-0], whereas all 12 strains were resistant to sulfamethoxazole [723-46-6]. All of these 12 strains were isolated and freeze-dried (1961-64) prior to clin. use of I. Of the 6 I-resistant strains, 5 were also resistant to tetracycline, chloramphenicol, and streptomycin. No evidence was found for R-factor involvement in I resistance. The experimental process involved the reaction of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate(cas: 23256-42-0).Reference of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate

The Article related to trimethoprim resistance klebsiella, sulfamethoxazole resistance klebsiella, Biochemical Interactions: Microbial Systems and other aspects.Reference of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Lu, Chen; Gutierrez-Bayona, Natalia Eugenia; Taylor, John-Stephen published an article in 2021, the title of the article was The effect of flanking bases on direct and triplet sensitized cyclobutane pyrimidine dimer formation in DNA depends on the dipyrimidine, wavelength and the photosensitizer.Application of 65-71-4 And the article contains the following content:

Cyclobutane pyrimidine dimers (CPDs) are the major products of DNA produced by direct absorption of UV light, and result in C to T mutations linked to human skin cancers. Most recently a new pathway to CPDs in melanocytes has been discovered that has been proposed to arise from a chemisensitized pathway involving a triplet sensitizer that increases mutagenesis by increasing the percentage of C-containing CPDs. To investigate how triplet sensitization may differ from direct UV irradiation, CPD formation was quantified in a 129-mer DNA designed to contain all 64 possible NYYN sequences. CPD formation with UVB light varied about 2-fold between dipyrimidines and 12-fold with flanking sequence and was most frequent at YYYR and least frequent for GYYN sites in accord with a charge transfer quenching mechanism. In contrast, photosensitized CPD formation greatly favored TT over C-containing sites, more so for norfloxacin (NFX) than acetone, in accord with their differing triplet energies. While the sequence dependence for photosensitized TT CPD formation was similar to UVB light, there were significant differences, especially between NFX and acetone that could be largely explained by the ability of NFX to intercalate into DNA. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Application of 65-71-4

The Article related to cyclobutane pyrimidine dimer photosensitizer wavelength photosensitization, Placeholder for records without volume info and other aspects.Application of 65-71-4

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Thirkell, D.; Blankson, M. published an article in 1981, the title of the article was The speciation of coliform genera from above and below a sewer outfall and their susceptibilities to antimicrobial agents.Recommanded Product: 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate And the article contains the following content:

The occurrence of coliforms in a small water course increased by a factor of 36 below the outfall of a sewage treatment plant. Speciation of the bacteria from above and below the sewer outfall showed that Escherichia coli and Enterobacter species predominated. Drug-resistance levels were significant in microorganisms from both sampling sites, and the occurrence of a significant number of multiple-resistant microorganisms, particularly E. coli, is reported. Both E. coli and Enterobacter species from below the sewer outfall showed a statistically significant increase in resistance to ampicillin [69-53-4] as compared with isolates from above the outfall, and E. coli from below the outfall also showed a statistically significant increase in resistance to sulphamethoxazole [723-46-6]. The experimental process involved the reaction of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate(cas: 23256-42-0).Recommanded Product: 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate

The Article related to coliform bacteria sewage antibiotic sensitivity, taxonomy coliform bacteria sewage, Biochemical Interactions: Microbial Systems and other aspects.Recommanded Product: 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On April 13, 2021, Pandey, Renu; Collins, Meghan; Lu, Xiyuan; Sweeney, Shannon R.; Chiou, Jennifer; Lodi, Alessia; Tiziani, Stefano published an article.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Novel Strategy for Untargeted Chiral Metabolomics using Liquid Chromatography-High Resolution Tandem Mass Spectrometry. And the article contained the following:

Stereospecific recognition of metabolites plays a significant role in the detection of potential disease biomarkers thereby providing new insights in diagnosis and prognosis. D-Hdroxy/amino acids are recognized as potential biomarkers in several metabolic disorders. Despite continuous advances in metabolomics technologies, the simultaneous measurement of different classes of enantiomeric metabolites in a single anal. run remains challenging. Here, we develop a novel strategy for untargeted chiral metabolomics of hydroxy/amine groups (-OH/-NH2) containing metabolites, including all hydroxy acids (HAs) and amino acids (AAs), by chiral derivatization coupled with liquid chromatog.-high resolution tandem mass spectrometry (LC-HR-MS/MS). Diacetyl-tartaric anhydride (DATAN) was used for the simultaneous derivatization of-OH/-NH2 containing metabolites as well as the resulting diastereomers, and all the derivatized metabolites were resolved in a single anal. run. Data independent MS/MS acquisition (DIA) was applied to pos. identify DATAN-labeled metabolites based on reagent specific diagnostic fragment ions. We discriminated chiral from achiral metabolites based on the reversal of elution order of D and L isomers derivatized with the enantiomeric pair (±) of DATAN in an untargeted manner. Using the developed strategy, a library of 301 standards that consisted of 214 chiral and 87 achiral metabolites were separated and detected in a single anal. run. This approach was then applied to investigate the enantioselective metabolic profile of the bone marrow (BM) and peripheral blood (PB) plasma samples from patients with acute myeloid leukemia (AML) at diagnosis and following completion of the induction phase of chemotherapeutic treatment. The sensitivity and selectivity of the developed method enabled the detection of trace levels of the D-enantiomer of HAs and AAs in primary plasma patient samples. Several of these metabolites were significantly altered in response to chemotherapy. The developed LC-HR-MS method entails a valuable step forward in chiral metabolomics. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to untargeted chiral metabolomics liquid chromatog high resolution mass spectrometry, tandem, Placeholder for records without volume info and other aspects.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On August 20, 2021, Peach, Jesse T.; Wilson, Stephanie M.; Gunderson, Logan D.; Frothingham, Lizzi; Tran, Tan; Walk, Seth T.; Yeoman, Carl J.; Bothner, Brian; Miles, Mary P. published an article.HPLC of Formula: 4433-40-3 The title of the article was Temporal metabolic response yields a dynamic biosignature of inflammation. And the article contained the following:

Chronic low-grade inflammation is a subclin. condition directly and indirectly linked to the development of a wide range of diseases responsible for the vast majority of morbidity. To examine mechanisms coupled to chronic disease, a group of overweight and obese human subjects without known inflammatory diseases participated in a high-fat meal challenge as an acute inflammation stimulus. Anal. of serum metabolites grouped by baseline cytokine levels revealed that single samples had little power in differentiating groups. However, an anal. that incorporated temporal response separated inflammatory response phenotypes and allowed us to create a metabolic signature of inflammation which revealed metabolic components that are crucial to a cytokine-mediated inflammation response. The use of temporal response, rather than a single time point, improved metabolomic prediction of high postprandial inflammation responses and led to the development of a dynamic biosignature as a potential tool for stratifying risk to a wide range of diseases. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).HPLC of Formula: 4433-40-3

The Article related to metabolic response biosignature inflammation, metabolomics, pathophysiology, systems biology, Placeholder for records without volume info and other aspects.HPLC of Formula: 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Grey, Daphne; Hamilton-Miller, J. M. T. published an article in 1977, the title of the article was Sensitivity of Pseudomonas aeruginosa to sulfonamides and trimethoprim and the activity of the combination trimethoprim: sulfamethoxazole.Computed Properties of 23256-42-0 And the article contains the following content:

P. aeruginosa strains from urinary infections were resistant or only moderately sensitive to sulfadiazine [68-35-9], sulfamethoxazole (I) [723-46-6], sulfadimidine [57-68-1], or trimethoprim lactate (II lactate) [23256-42-0], but a marked synergy between I and II was noted with the moderately sensitive strains. Thus, therapeutically attainable urinary levels of these drugs (which were ineffective individually) were quite inhibitory to P. aeruginosa when used in combination. The min. inhibitory concentrations of I and II when used in combination suggested that disks containing I and II at a 1:2 ratio would be more appropriate for determining the drug susceptibility of urinary pathogens than the usual 1:20 ratio. The experimental process involved the reaction of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate(cas: 23256-42-0).Computed Properties of 23256-42-0

The Article related to sulfonamide trimethoprim pseudomonas sensitivity, urine pseudomonas trimethoprim sulfamethoxazole, Biochemical Interactions: Microbial Systems and other aspects.Computed Properties of 23256-42-0

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On May 25, 2018, Woodring, Jennifer L.; Bachovchin, Kelly A.; Brady, Kimberly G.; Gallerstein, Mitchell F.; Erath, Jessey; Tanghe, Scott; Leed, Susan E.; Rodriguez, Ana; Mensa-Wilmot, Kojo; Sciotti, Richard J.; Pollastri, Michael P. published an article.Category: pyrimidines The title of the article was Corrigendum to “Optimization of physicochemical properties for 4-Anilinoquinazoline inhibitors of trypanosome proliferation” [Eur. J. Med. Chem. 141 (2017) 446-459] [Erratum to document cited in CA167:595582]. And the article contained the following:

In the original publication, the acknowledgments section has information omitted; the correction is provided here. The experimental process involved the reaction of 4-(2-Bromopyrimidin-4-yl)morpholine(cas: 1209459-32-4).Category: pyrimidines

The Article related to anilinoquinazoline trypanosome inhibitor antimalarial malaria trypanosomiasis trypanosomicide erratum, Placeholder for records without volume info and other aspects.Category: pyrimidines

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On October 31, 2019, Rus, Lucia Maria; Donici, Elena; Valica, Vladimir; Prisacari, Viorel; Tomuta, Ioan; Sepeli, Diana; Heghes, Simona Codruta; Iuga, Cristina Adela; Uncu, Livia published an article.Safety of 6-Methylpyrimidine-2,4(1H,3H)-dione The title of the article was Development, physical-chemical characterization and in vitro antibacterial activity evaluation of a fixed-dose combination isohydrafural-methyluracil hydrophilic ointment. And the article contained the following:

This research was focused on the pharmaceutical development of a novel, fixed-dose combination isohydrafural-methyluracil ointment on a water soluble base – polyethylene glycol (PEG). Differential scanning calorimetry (DSC) and Fourier transform infra-red spectroscopy (FTIR) were applied in order to determine the compatibility between active pharmaceutical ingredients (APIs) and between APIs – excipients. Nine formulations (F1 – F9) were tested for: phys. appearance and homogeneity, pH, viscosity and osmotic activity. The optimal formulation presented the concentrations of PEG 400 – 76.72%, PEG 1500 – 19.18% and a fixed-dose combination of 0.1% isohydrafural and 4% methyluracil. It showed higher antibacterial properties both to Gram-pos. and Gram-neg. bacteria than nitrofural 2% ointment (Furacilin, Belarus). The long-term stability testing of the optimized formulation of isohydrafural and methyluracil revealed the shelf-life of two years. The experimental process involved the reaction of 6-Methylpyrimidine-2,4(1H,3H)-dione(cas: 626-48-2).Safety of 6-Methylpyrimidine-2,4(1H,3H)-dione

The Article related to staphylococcus enterococcus escherichia klebsiella pseudomonas proteus isohydrafural methyluracil antibacterial, Placeholder for records without volume info and other aspects.Safety of 6-Methylpyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Krell, Katja; Achim-Wagenknecht, Hans published an article in 2020, the title of the article was Fluorogenic and bioorthogonal modification of RNA using photoclick chemistry.Computed Properties of 4433-40-3 And the article contains the following content:

A bromoaryltetrazole-modified uridine was synthesized as new RNA building block for bioorthogonal, light-activated and postsynthetic modification with com. available fluorescent dyes. It allows “photoclick”-type modifications by irradiation with light (300 nm LED) at internal and terminal positions of presynthesized RNA with maleimide-conjugated fluorophores in good yields. The reaction was evidenced for three different dyes. During irradiation, the emission increases due to the formation of an intrinsically fluorescent pyrazoline moiety as photoclick product. The fluorogenecity of the photoclick reaction was significantly enhanced by energy transfer between the pyrazoline as the reaction product (poor emitter) and the photoclicked dye as the strong emitter. The RNA-dye conjugates show remarkable fluorescent properties, in particular an up to 9.4 fold increase of fluorescence, which are important for chem. biol. and fluorescent imaging of RNA in cells. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Computed Properties of 4433-40-3

The Article related to phosphoramidite tetrazole rna fluorescent bioorthogonal photoclick chem, oligonucleotide, photochemistry, tetrazole, Placeholder for records without volume info and other aspects.Computed Properties of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On June 30, 2017, Podesvova, Lucie; Huang, Huan; Yurchenko, Vyacheslav published an article.Quality Control of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate The title of the article was Inducible protein stabilization system in Leishmania mexicana. And the article contained the following:

Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD. The experimental process involved the reaction of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate(cas: 23256-42-0).Quality Control of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate

The Article related to protein stabilization system leishmania dihydrofolate reductase, leishmania mexicana, protein stabilization, trimethoprim, ecdhfr, Enzymes: Structure-Conformation-Active Site and other aspects.Quality Control of 5-(3,4,5-Trimethoxybenzyl)pyrimidine-2,4-diamine 2-hydroxypropanoate

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia