Day: November 9, 2022

Equine herpes virus 1 and pseudorabies virus resistance to 2′-fluoropyrimidine analogs and to bromovinyldeoxyuridine: implications for dTMP kinase activity was written by Veerisetty, V.; Balasubramaniam, N. K.; Gentry, G. A.. And the article was included in Acta Virologica (English Edition) on December 31,1990.Category: pyrimidines The following contents are mentioned in the article:

The 2′-fluoropyrimidine nucleoside analogs 1-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)-5-iodocytosine (FIAC), 1-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)-5-methyluracil (FMAU), and 1-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)-5-iodouracil (FIAU) showed higher in vitro activity against herpes simplex virus type 1 (HSV-1) than equine herpesvirus 1 (EHV-1) or pseudorabies virus (PRV). Comparison of the 50% plaque inhibitory doses for HSV-1 and its mutant MMdUr-20 in cell cultures with inhibition constants for the viral deoxythymidine kinases (dTKs) suggests that in the infected cell FMAU is phosphorylated by host enzymes. As compared to HSV-1, EHV-1 and PRV were more resistant to E-5-(2-bromovinyl)-2′-deoxyuridine (BVdU) and to the 2′-fluoropyrimidine analogs, as are HSV-2 and the HSV-1 mutants MMdUr-20 and S1. Because the dTKs of the latter lack deoxythymidylate kinase (dTMPK) activity, there appears to be a correlation between resistance to these analogs and BVdU on the one hand, and lack of dTMPK activity on the other. EHV-1 and PRV dTKs lack significant dTMPK activity. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Category: pyrimidines).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. Pyrimidine also found in many synthetic compounds such as barbiturates and the HIV drug, zidovudine. Pyrimidine derivatives also play an important role in drug development, either in concert with other compounds or on their own.Category: pyrimidines

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3

Development and validation of stability indicating HPLC method for the estimation of Minoxidil and related substance in topical formulation was written by Gaidhane, Hemant K.; Bidada, Jagdish P.; Bhusari, Akshay S.; Navkhare, Manjusha S.; Diwanka, Ganesh P.; Tiwari, Ashish H.. And the article was included in Journal of Pharmacy Research on December 31,2011.SDS of cas: 35139-67-4 The following contents are mentioned in the article:

A sensitive HPLC method was developed and validated for the determination of minoxidil and related substances in a novel topical formulation. The highly polar mol. requires aqueous mobile phase for the elution and separation of Minoxidil and its impurities (Impurity A, B and E). The developed method is found to be specific, reproducible and stability indicating. The Phenomenex Synergi Polar RP 250 × 4.6 mm 4μ column was used and mobile phase contains 2.0mL of di-Et amine and 40mL of glacial acetic acid in 1000mL of milli Q water (Buffer) and 20% THF in methanol (mobile phase A & B resp.) to achieve good resolution and retention of the analyte and its related impurities. The detector linearity was established from concentrations ranging from 0.02-400 μg/mL for Minoxidil and from 0.02-1.0 μg/mL for related substances with a correlation co-efficient of 0.999. The relative response factor (RRF) values of impurity A, impurity B and impurity E determined from linearity plots were 0.24, 0.31 and 1.07 resp. The limit of detection (LOD) and limit of quantification (LOQ) found to be 0.02μg/mL and 0.1μg/mL resp. for Minoxidil and related substances. The mol. was stable in all the stress conditions such as acid, base, heat photolysis and unstable in oxidation as per the recommendations of ICH guidelines. The method was proved to be robust with respect to changes in flow rate, pH and column temperature This study involved multiple reactions and reactants, such as 2,6-Diamino-4-chloropyrimidine-1-oxide (cas: 35139-67-4SDS of cas: 35139-67-4).

2,6-Diamino-4-chloropyrimidine-1-oxide (cas: 35139-67-4) belongs to pyrimidine derivatives. The pyrimidine derivatives can easily interact with enzymes, genetic materials, and bio components within the cell. For example, the neurotoxin tetrodotoxin is a pyrimidine derivative. It is found in a number of species including the Japanese puffer fish, the blue-ringed octopus, and the orange-bellied newt. Tetrodotoxin prevents the transmission of nerve signals and can result in paralysis and death.SDS of cas: 35139-67-4

35139-67-4;2,6-Diamino-4-chloropyrimidine-1-oxide;The future of 35139-67-4;New trend of C4H5ClN4O;function of 35139-67-4

Human cytomegalovirus-induced DNA polymerase and its interaction with the triphosphates of 1-(2′-deoxy-2′-fluoro-β-D-arabinofuranosyl)-5-methyluracil, -5-iodocytosine, and -5-methylcytosine was written by Mar, Eng Chun; Chiou, Jwo Farn; Cheng, Yung Chi; Huang, Eng Shang. And the article was included in Journal of Virology on December 31,1985.Computed Properties of C10H13FN2O5 The following contents are mentioned in the article:

Human cytomegalovirus-induced DNA polymerase  [9012-90-2] and cellular DNA polymerase α were purified by successive chromatog. on DEAE-cellulose, phosphocellulose, heparin agarose, and single-stranded DNA agarose columns. The purified virus-induced DNA polymerase was resolved to 2 polypeptides corresponding to mol. weights of 140,000 and 58,000, as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Virus-induced DNA polymerase and cellular α polymerase were examined for their sensitivities to the triphosphates of 1-(2′-deoxy-2′-fluoro-β-D-arabinofuranosyl)-5-methyluracil (FMAUTP) [69256-17-3], -5-iodocytosine (FIACTP) [69123-90-6], and -5-methylcytosine (FMACTP) [78636-53-0]. The inhibitive effects of these triphosphates on the DNA polymerases were competitive with regard to the natural substrates; thus FMAUTP completes with dTTP  [365-08-2], and FIACTP and FMACTP complete with dCTP  [2056-98-6]. The inhibition constants (Ki) for FMAUTP, FIACTP, and FMACTP of virus-induced DNA polymerase are 0.06, 0.30, and 0.47 μM, resp. Cellular DNA polymerase α is much less sensitive to these inhibitors, and its Ki values for FMAUTP, FIACTP, and FMACTP are 0.45, 3.10, and 2.90 μM, resp. In addition, human cytomegalovirus-induced DNA polymerase, but not cellular DNA polymerase α, can utilize these analog triphosphates as alternate substrates for their corresponding natural deoxyribonucleoside triphosphates in in vitro DNA synthesis. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Computed Properties of C10H13FN2O5).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. Pyrimidine is an aromatic heterocyclic organic compound similar to pyridine. As nucleotides in DNA and RNA, pyrimidine nucleotide derivatives have a wide range of biological applications. For example, pyrimidine derivatives are useful in DNA repair studies involving cancer and epigenetics.Computed Properties of C10H13FN2O5

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3

Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme was written by Kosinska, Urszula; Carnrot, Cecilia; Eriksson, Staffan; Wang, Liya; Eklund, Hans. And the article was included in FEBS Journal on December 31,2005.Safety of 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione The following contents are mentioned in the article:

Thymidine kinases have been found in most organisms, from viruses and bacteria to mammals. Ureaplasma urealyticum (parvum), which belongs to the class of cell-wall-lacking Mollicutes, has no de novo synthesis of DNA precursors and therefore has to rely on the salvage pathway. Thus, thymidine kinase (Uu-TK) is the key enzyme in dTTP synthesis. Recently the 3D structure of Uu-TK was determined in a feedback inhibitor complex, demonstrating that a lasso-like loop binds the thymidine moiety of the feedback inhibitor by hydrogen bonding to main-chain atoms. Here the structure with the substrate deoxythymidine is presented. The substrate binds similarly to the deoxythymidine part of the feedback inhibitor, and the lasso-like loop binds the base and deoxyribose moieties as in the complex determined previously. The catalytic base, Glu-97, has a different position in the substrate complex from that in the complex with the feedback inhibitor, having moved in closer to the 5′-OH of the substrate to form a hydrogen bond. The phosphorylation of and inhibition by several nucleoside analogs were investigated and are discussed in the light of the substrate binding pocket, in comparison with human TK1. Kinetic differences between Uu-TK and human TK1 were observed that may be explained by structural differences. The tight interaction with the substrate allows minor substitutions at the 3 and 5 positions of the base, only fluorine substitutions at the 2′-Ara position, but larger substitutions at the 3′ position of the deoxyribose. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Safety of 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. The pyrimidine derivatives can easily interact with enzymes, genetic materials, and bio components within the cell. Pyrimidine derivatives also play an important role in drug development, either in concert with other compounds or on their own.Safety of 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3

Hydrocephalus induction in mice infected with herpes simplex virus type 2 after antiviral treatment was written by Schinazi, Raymond F.; Yao, Xuejun. And the article was included in Antiviral Research on December 31,1995.Product Details of 69256-17-3 The following contents are mentioned in the article:

By using antiviral chemotherapy to moderate the lethal effect of wild-type herpes simplex virus type 2 (HSV-2), a new mouse model for herpes simplex virus (HSV)-induced hydrocephalus was developed. Groups of BALB/c mice were infected either intracerebrally (i.c.) or i.p. with a LD of HSV-2. The antiviral agent 2′-fluoro-5-methylarabinosyluracil (FMAU) was administered i.p. 2 days after virus inoculation. By day 21, 80 and 71.4% of the mice infected i.c. or i.p., resp., survived. The surviving animals were randomly subdivided into different groups and some were challenged i.c. or i.p. with a lethal or super-LD of homologous virus. The mice were sacrificed at 2 or 3 mo after the initial virus infection. Neuropathol. changes of the brains were assessed. Dilation of lateral and third ventricles was noted in the animals initially inoculated i.c., especially in all the animals inoculated i.c. and challenged i.c. with a super-lethal virus inoculum, but not in those inoculated i.p. Microscopic examination of hydrocephalic brains revealed evidence of viral meningoencephalitis. Two different mechanisms of ventricular enlargement in this animal model are proposed. This model is relevant since HSV-induced cases of hydrocephalus have been reported to occur in humans and in particular neonates. Issues of virus persistence and expression, long-term evaluation for disease progression, and intervention strategies could be examined with this model. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Product Details of 69256-17-3).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. The aromatic compound pyrimidine, and its derivatives, are ubiquitous in nature. They are found in nucleic acids, vitamins, amino acids, antibiotics, alkaloids, and a variety of toxins. Pyrimidine derivatives also play an important role in drug development, either in concert with other compounds or on their own.Product Details of 69256-17-3

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3

Monitoring tumor cell proliferation by targeting DNA synthetic processes with thymidine and thymidine analogs was written by Schwartz, Jeffrey L.; Tamura, Yasuko; Jordan, Robert; Grierson, John R.; Krohn, Kenneth A.. And the article was included in Journal of Nuclear Medicine on December 31,2003.Recommanded Product: 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione The following contents are mentioned in the article:

The use of radiolabeled thymidine (TdR) and thymidine analogs as PET-based tracers of tumor growth rate is based on the assumption that measurement of uptake of these nucleosides, a function primarily of thymidine kinase-1 (TK1) activity, provides an accurate measure of active cell proliferation in tumors. The goal of this study was to test this hypothesis and determine how well these tracers track changes in proliferation of tumor cells. Methods: TK1 activity; S-phase fraction; and uptake of TdR, 3′-deoxy-3′-fluorothymidine (FLT), and 2′-fluoro-5-methyl-1-(β-D-2-arabino-furanosyl) uracil (FMAU) were determined in plateau-phase and exponentially growing cultures of 3 human and 3 murine tumor cell lines. Results: TK1 activity and S-phase fraction increased in all cell lines as cells moved from plateau-phase conditions to exponential growth. Some cell lines had relatively large TK1 activities and S-phase fractions under plateau-phase conditions, consistent with a loss of normal cell cycle checkpoint control in these cells. There were also 2 cell lines in which TK1 activity changed little as cells moved from the plateau phase to exponential growth, suggesting that in these cell lines, de novo nucleotide synthesis pathways predominate over salvage pathways. Both TdR and FLT detected changes in TK1 activity. The slope of the relationship between TdR uptake and TK1 activity was nearly twice that for FLT and more than 40-fold that for FMAU. Conclusion: Although not all tumors show a strong TK1 dependence of proliferation, in all cell lines for which proliferation is highly TK1 dependent, phosphorylation of TdR or FLT accurately reflects changes in TK1 enzyme activity. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Recommanded Product: 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. Pyrimidine is an aromatic heterocyclic organic compound similar to pyridine. Pyrimidine derivatives also play an important role in drug development, either in concert with other compounds or on their own.Recommanded Product: 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3

Incorporation and metabolism of 2′-fluoro-5-substituted arabinosyl pyrimidines and their selective inhibition of viral DNA synthesis in herpes simplex virus type 1 (HSV-1)-infected and mock-infected Vero cells was written by Kong, Xiang Bin; Scheck, Adrienne C.; Price, Richard W.; Vidal, Pedro M.; Fanucchi, Michael P.; Watanabe, Kyoichi A.; Fox, Jack J.; Chou, Ting Chao. And the article was included in Antiviral Research on December 1,1988.Synthetic Route of C10H13FN2O5 The following contents are mentioned in the article:

The incorporation and metabolism of 2′-fluoro-5-substituted arabinosyl pyrimidine analogs I and II (R = H, I, Me, Et, or CHF2) and their selective inhibition of viral DNA synthesis in herpes simplex virus type 1 (HSV-1)-infected and mock-infected Vero cells were studied by HPLC and CsCl isopycnic d. gradient anal. of isolated DNAs. The amounts of radiolabeled analogs incorporated as parent compound following 10 μM exposure for 4 h were 10-fold higher in HSV-1-infected vs. mock-infected cells for 2′-fluoro-5-difluoromethyl-Ara-U (F2FMAU); 4.3-fold higher for 5-Et deoxyuridine (EdU); 2.6-fold higher for 2′-fluoro-5-methyl-Ara-U (FMAU) and 1.7-fold higher for dThd. For 2′-fluoro-5-ethyl-Ara-U (FEAU), 3.0 pmole of unchanged moiety was incorporated per 106 HSV-1-infected cells but no incorporation was detected in mock-infected cells. HPLC profiles showed that the percentages of radiolabeled analogs incorporated as parent compound in the DNA extracted from HSV-1-infected cells were 31.0% for F2FMAU, 99.6% for EdU, 83.5% for FEAU and 98.3% for FMAU; from mock-infected cells, they were 63.6% for F2FMAU, 96.7% for EdU, 97.3% for FMAU and no incorporation into DNA for FEAU was detected. CsCl d. gradient analyses of isolated DNA showed that viral DNA synthesis was inhibited 98% by 10 μM FEAU, 92% by 10 μM F2FMAU, 90% by 2 μM FMAU and 80% by 50 μM EdU, whereas cellular DNA synthesis was inhibited by 53, 44, 61, 66 and 54%, resp. It was shown that: (a) FEAU incorporation into host-cell DNA was not detectable but FEAU was selectively incorporated into HSV-infected cells; (b) FMAU and FEAU were metabolically stable; however, F2FMAU was extensively metabolized; (c) FEAU and F2FMAU were among the most selective inhibitors of HSV-1 DNA synthesis while allowing cellular DNA synthesis to continue. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Synthetic Route of C10H13FN2O5).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. The pyrimidine nitrogenous bases are derived from the organic compound pyrimidine through the addition of various functional groups. As nucleotides in DNA and RNA, pyrimidine nucleotide derivatives have a wide range of biological applications. For example, pyrimidine derivatives are useful in DNA repair studies involving cancer and epigenetics.Synthetic Route of C10H13FN2O5

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3

Incorporation and metabolism of 2′-fluoro-5-substituted arabinosyl pyrimidines and their selective inhibition of viral DNA synthesis in herpes simplex virus type 1 (HSV-1)-infected and mock-infected Vero cells was written by Kong, Xiang Bin; Scheck, Adrienne C.; Price, Richard W.; Vidal, Pedro M.; Fanucchi, Michael P.; Watanabe, Kyoichi A.; Fox, Jack J.; Chou, Ting Chao. And the article was included in Antiviral Research on December 1,1988.COA of Formula: C9H12FN3O4 The following contents are mentioned in the article:

The incorporation and metabolism of 2′-fluoro-5-substituted arabinosyl pyrimidine analogs I and II (R = H, I, Me, Et, or CHF2) and their selective inhibition of viral DNA synthesis in herpes simplex virus type 1 (HSV-1)-infected and mock-infected Vero cells were studied by HPLC and CsCl isopycnic d. gradient anal. of isolated DNAs. The amounts of radiolabeled analogs incorporated as parent compound following 10 μM exposure for 4 h were 10-fold higher in HSV-1-infected vs. mock-infected cells for 2′-fluoro-5-difluoromethyl-Ara-U (F2FMAU); 4.3-fold higher for 5-Et deoxyuridine (EdU); 2.6-fold higher for 2′-fluoro-5-methyl-Ara-U (FMAU) and 1.7-fold higher for dThd. For 2′-fluoro-5-ethyl-Ara-U (FEAU), 3.0 pmole of unchanged moiety was incorporated per 106 HSV-1-infected cells but no incorporation was detected in mock-infected cells. HPLC profiles showed that the percentages of radiolabeled analogs incorporated as parent compound in the DNA extracted from HSV-1-infected cells were 31.0% for F2FMAU, 99.6% for EdU, 83.5% for FEAU and 98.3% for FMAU; from mock-infected cells, they were 63.6% for F2FMAU, 96.7% for EdU, 97.3% for FMAU and no incorporation into DNA for FEAU was detected. CsCl d. gradient analyses of isolated DNA showed that viral DNA synthesis was inhibited 98% by 10 μM FEAU, 92% by 10 μM F2FMAU, 90% by 2 μM FMAU and 80% by 50 μM EdU, whereas cellular DNA synthesis was inhibited by 53, 44, 61, 66 and 54%, resp. It was shown that: (a) FEAU incorporation into host-cell DNA was not detectable but FEAU was selectively incorporated into HSV-infected cells; (b) FMAU and FEAU were metabolically stable; however, F2FMAU was extensively metabolized; (c) FEAU and F2FMAU were among the most selective inhibitors of HSV-1 DNA synthesis while allowing cellular DNA synthesis to continue. This study involved multiple reactions and reactants, such as 4-Amino-1-((2R,3S,4R,5R)-3-fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidin-2(1H)-one (cas: 56632-83-8COA of Formula: C9H12FN3O4).

4-Amino-1-((2R,3S,4R,5R)-3-fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidin-2(1H)-one (cas: 56632-83-8) belongs to pyrimidine derivatives. The aromatic compound pyrimidine, and its derivatives, are ubiquitous in nature. They are found in nucleic acids, vitamins, amino acids, antibiotics, alkaloids, and a variety of toxins. We all know its importance to life – pyrimidine and purine bases are included in the structure of DNA and RNA.COA of Formula: C9H12FN3O4

56632-83-8;4-Amino-1-((2R,3S,4R,5R)-3-fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidin-2(1H)-one;The future of 56632-83-8;New trend of C9H12FN3O4;function of 56632-83-8

Toxicity, metabolism, DNA incorporation with lack of repair, and lactate production for 1-(2′-fluoro-2′-deoxy-β-D-arabinofuranosyl)-5-iodouracil in U-937 and MOLT-4 cells was written by Klecker, Raymond W.; Katki, Aspandiar; Collins, Jerry M.. And the article was included in Molecular Pharmacology on December 31,1994.Synthetic Route of C10H13FN2O5 The following contents are mentioned in the article:

Two cell lines, U-937 and MOLT-4, were used to investigate the toxicity, DNA incorporation, and effect on mitochondria of 1-(2′-fluoro-2′-deoxy-β-D-arabinofuranosyl)-5-iodouracil (FIAU) and its putative metabolite 1-(2′-fluoro-2′-deoxy-β-D-arabinofuranosyl)uracil (FAU). After 72-h incubation, the IC50 values for FIAU were 6.4 μM for U-937 cells and 26 μM for MOLT-4 cells. IC50 values for FAU were 10-fold higher in both cell lines. Incubation for 24 h with 10 μM [2-14C]FIAU led to 2.1% and 0.93% replacement of thymidine in DNA of U-937 and MOLT-4 cells, resp. The predominant radioactive species measurable in DNA was FIAU. A similar incubation with [2-14C]FAU resulted in 4-fold lower DNA incorporation of a single radioactive species that coeluted with 1-(2′-fluoro-2′-deoxy-β-D-arabinofuranosyl)-5-methyluracil (FMAU). There was no evidence of a selective repair process after DNA incorporation of FIAU or FAU (FMAU). Increased intracellular concentrations of FIAU triphosphate and incorporation into DNA were associated with an increase in cellular toxicity. Continuous exposure to a clin. achievable concentration of FIAU, 0.44 μM, produced a constant DNA incorporation of 0.80% and 0.11% for U-937 and MOLT-4 cells, resp. FIAU was not readily metabolized to FAU or iodouracil by human liver in vitro. Compared with 2′,3′-dideoxycytidine as a pos. control, after 12 days of continuous exposure of U-937 and MOLT-4 cells to FIAU there was no evidence of increased lactate production These data negate several possible mechanisms of FIAU hepatotoxicity(DNA chain termination, DNA polymerase inhibition, one form of selective mitochondrial poisoning, and FAU-mediated toxicity) and provide clues for possible other mechanisms (FIAU triphosphate concentration and DNA incorporation). Further work is needed to develop a complete explanation for the delayed hepatic toxicity observed in the investigational clin. trials of FIAU. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Synthetic Route of C10H13FN2O5).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. Pyrimidines are isomeric with two other forms of diazines: pyridazine, with the nitrogen atoms in the 1 and 2 positions; and pyrazine, with the nitrogen atoms in the 1 and 4 positions. We all know its importance to life – pyrimidine and purine bases are included in the structure of DNA and RNA.Synthetic Route of C10H13FN2O5

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3

Drug resistance patterns of herpes simplex virus isolates from patient treated with acyclovir was written by McLaren, Colin; Chen, Ming S.; Ghazzouli, Ismail; Saral, Rein; Burns, William H.. And the article was included in Antimicrobial Agents and Chemotherapy on December 31,1985.Synthetic Route of C10H13FN2O5 The following contents are mentioned in the article:

A decrease in the in vitro sensitivity to acyclovir (ACV) [59277-89-3] was observed in successive isolates of herpes simplex virus type 1 from immunocompromised patients during i.v. therapy with this drug. The ACV-resistant isolate from patient 1 was cross-resistant to 9-(1,3-dihydroxy-2-propoxymethyl)guanine  [82410-32-0] and (E)-5-(2-bromovinyl-2′-deoxyuridine  [69304-47-8], but still susceptible to 3 fluoro-substituted pyrimidines, 2′-fluoro-5-iodo-1-β-D-arabinofuranosylcytosine (FIAC) [69123-90-6], 2′-fluoro-5-iodo-1-β-D-arabinofuranosyluracil (FIAU) [69123-98-4] and 2′-fluoro-1-β-D-arabinofuranosylthymine (FMAU) [69256-17-3]. Thymidine kinase (TK) [9002-06-6] from the resistant isolate showed a 50-fold or greater reduction in affinity for thymidine, FIAU, FMAU, and ACV, but the total enzyme activity was similar to that of the sensitive isolate. The ACV-resistant isolate from patient 2 was also resistant to the dihydroxypropyoxymethylguanine, the bromovinyldeoxyuridine, and the fluoro-substituted compounds; TK activity for this isolate was <1% of the patient's pretherapy isolate. An isolate obtained during a subsequent recurrence in patient 2 was susceptible to ACV and the other TK-dependent agents. The ACV-resistant isolate from patient 3 was partially resistant to FIAC and FIAU but still susceptible to FMAU; the viral TK had a 10-fold-lower affinity for ACV, FIAU, and FMAU than did the sensitive pretherapy isolate, whereas the level of TK activity detected was reduced to 6%. In none of the isolates studied was a change in sensitivity to phosphonoformic acid observed Compared with the corresponding pretherapy ACV-sensitive isolates, there was a 30-fold decrease in neurovirulence for mice of the 2 drug-resistant isolates with diminished levels of thymidine-phosphorylating activity and no change in virulence for the 3rd isolate. These findings indicate that mixed patterns of drug-resistance to TK-dependent antiviral compounds can occur in clin. isolates, resulting from changes in either the amount or the affinity of viral TK activity. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Synthetic Route of C10H13FN2O5).

1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. The aromatic compound pyrimidine, and its derivatives, are ubiquitous in nature. They are found in nucleic acids, vitamins, amino acids, antibiotics, alkaloids, and a variety of toxins. Drugs having the pyrimidine motif have manifested to exhibit gratifying biological activity like anticancer, antiviral, anti-inflammatory, antibacterial, and antihypertensive activities.Synthetic Route of C10H13FN2O5

69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3