Kim, Ju Yeong; Yi, Myung-Hee; Kim, Myungjun; Yeom, Joon-Sup; Yoo, Hyun Dong; Kim, Seong Min; Yong, Tai-Soon published an article in 2022, the title of the article was Diagnosis of Balamuthia mandrillaris encephalitis by thymine-adenine cloning using universal eukaryotic primers.Recommanded Product: 5-Methylpyrimidine-2,4(1H,3H)-dione And the article contains the following content:
Identifying the causal pathogen of encephalitis remains a clin. challenge. A 50-yr-old man without a history of neurol. disease was referred to our department for the evaluation of an intracranial lesion observed on brain magnetic resonance imaging (MRI) scans, and the pathol. results suggested protozoal infection. We identified the species responsible for encephalitis using thymine-adenine (TA) cloning, suitable for routine clin. practice. We extracted DNA from a paraffin-embedded brain biopsy sample and performed TA cloning using two universal eukaryotic primers targeting the V4-5 and V9 regions of the 18S rRNA gene. The recombinant plasmids were extracted, and the inserted amplicons were identified by Sanger sequencing and a homol. search of sequences in the National Center for Biotechnol. Information Basic Local Alignment Search Tool. The infection was confirmed to be caused by the free-living amoeba Balamuthia mandrillaris. Two of 41 colonies recombinant with 18S V4-5 primers and 35 of 63 colonies recombinant with the 18S V9 primer contained B. mandrillaris genes; all other colonies contained human genes. Pathogen-specific PCR ruled out Entamoeba histolytica, Naegleria fowleri, Acanthamoeba spp., and Toxoplasma gondii infections. This is the first report of B. mandrillaris-induced encephalitis in Korea based on mol. identification. TA cloning with the 18S rRNA gene is a feasible and affordable diagnostic tool for the detection of infectious agents of unknown etiol. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Recommanded Product: 5-Methylpyrimidine-2,4(1H,3H)-dione
The Article related to thymine adenine balamuthia mandrillaris encephalitis infection diagnosis, 18s rrna, amoeba, balamuthia mandrillaris, encephalitis, ta cloning, Mammalian Pathological Biochemistry: Nervous System and Psychiatric Diseases and other aspects.Recommanded Product: 5-Methylpyrimidine-2,4(1H,3H)-dione
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