Bohacova, Sona; Ludvikova, Lucie; Postova Slavetinska, Lenka; Vanikova, Zuzana; Klan, Petr; Hocek, Michal published an article in 2018, the title of the article was Protected 5-(hydroxymethyl)uracil nucleotides bearing visible-light photo-cleavable groups as building blocks for polymerase synthesis of photo-caged DNA.Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione And the article contains the following content:
Nucleosides, nucleotides and 2′-deoxyribonucleoside triphosphates (dNTPs) containing 5-(hydroxymethyl)uracil protected with photo-cleavable groups (2-nitrobenzyl-, 6-nitropiperonyl or 9-anthrylmethyl) were prepared and tested as building blocks for the polymerase synthesis of photo-caged oligonucleotides and DNA. Photo-deprotection (photo-release) reactions were studied in detail on model nucleoside monophosphates and their photoreaction quantum yields were determined Photo-caged dNTPs were then tested and used as substrates for DNA polymerases in primer extension or PCR. DNA probes containing photo-caged or free 5-hydroxymethyluracil in the recognition sequence of restriction endonucleases were prepared and used for the study of photo-release of caged DNA by UV or visible light at different wavelengths. The nitropiperonyl-protected nucleotide was found to be a superior building block because the corresponding dNTP is a good substrate for DNA polymerases, and the protecting group is efficiently cleavable by irradiation by UV or visible light (up to 425 nm). The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
The Article related to hydroxymethyluracil nucleotide preparation photocleavable polymerase photocaged dna, Carbohydrates: Nucleosides and Nucleotides, Cobalamins, Riboflavin and other aspects.Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia