On January 31, 2022, Khattab, Reham Abdel-Halim; Barghash, Safaa Mohamed; Mostafa, Osama Mohammad Sayed; Allam, Sahar Ali; Taha, Hoda Abdel-Halim; Ashour, Ameen Abd El-Baqi published an article.HPLC of Formula: 65-71-4 The title of the article was Seroprevalence and molecular characterization of Toxoplasma gondii infecting ruminants in the North-West of Egypt. And the article contained the following:
Toxoplasma gondii is a coccidian parasite known for its heavy toll on people and livestock. It can cause abortion and a variety of congenital diseases. The current study aimed to examine some seroprevalence and mol. attributes of T. gondii obtained from ruminants in the North-West of Egypt. Specimens were random selected from five different locations in Alexandria and Matrouh governorates. A total of 483 blood samples, collected from 96 mixed flocks, were screened for anti-T. gondii IgG antibodies using ELISA (ELISA). The seropos. results were then confirmed using polymerase chain reaction (PCR) primers for the B1 and P30 genes. Specific PCR products were selected for sequencing and alignment against the GenBank, where phylogeny has been examined using the maximum likelihood, neighbor-joining, and maximum parsimony in MEGA6. ELISA confirmed the presence of T. gondii in 188 of the investigated samples (38.92%), indicating a higher prevalence in camels (64.51%) and sheep (43.75%) as compared to goats (27.93%) and cattle (13.46%). PCR confirmed the presence of T. gondii-specific sequences in 159 seropos. specimens, with homol. between 98.3 and 100%. The genetic distances between the investigated variants ranged from 0.1 to 0.9, and 7 single nucleotide polymorphisms (SNPs), were identified in the examined T. gondii specimens. The camel T. gondii parasite, isolated from Matrouh, showed a 100% homol. with the most dangerous reference strains of T. gondii-RH in the GenBank. Our results showed that B1 and P30-specific PCR could detect T. gondii in blood samples more accurately than ELISA. In addition, the statistical anal. of our data indicated that species, age, sex, and animal location were all risk factors for toxoplasmosis. These findings are likely to boost disease control and help contain the spread of T. gondii infections. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).HPLC of Formula: 65-71-4
The Article related to igg b1 p30 guanine thymine toxoplasma infection, b1gene, elisa, egypt, p30 gene, ruminants, toxoplasma gondii, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.HPLC of Formula: 65-71-4
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia