On May 5, 2020, Herl, Thomas; Matysik, Frank-Michael published an article.Electric Literature of 65-71-4 The title of the article was Investigation of the Electrooxidation of Thymine on Screen-Printed Carbon Electrodes by Hyphenation of Electrochemistry and Mass Spectrometry. And the article contained the following:
The electrooxidation of thymine on screen-printed C electrodes was studied using different complementary instrumental approaches. The potential-dependent product profile was obtained by recording real-time mass voltammograms. Electrochem. flow cells with integrated disposable electrodes were directly coupled with mass spectrometry to facilitate a very fast detection of electrogenerated species. Thymine dimers were found at a potential of ∼1.1 V in ammonium acetate (pH 7.0) and 1.25 V in ammonium H carbonate electrolyte (pH 8.0). Electrochem.-capillary electrophoresis-mass spectrometry measurements revealed that two isobaric isomers of a dimeric oxidation product were formed. Separations at different time intervals between end of oxidation and start of separation showed that these were hydrated over time. A study of the pKa values by changing the separation conditions in electrochem.-capillary electrophoresis-UV-visible spectroscopy measurements allowed for further characterization of the primary oxidation products. Both isomers exhibited two deprotonation steps. The oxidation products were further characterized by HPLC-tandem mass spectrometry. Based on the obtained data, the main oxidation products of thymine in aqueous solution could most likely be identified as N(1)-C(5′) and N(1)-C(6′) linked dimer species evolving into the corresponding dimer hydrates over time. The presented methods for online characterization of electrochem. pretreated samples showed that not only mass spectrometric data can be obtained by electrochem.-mass spectrometry but also further characterizations such as the study of product stability and the pH-dependent protonation or deprotonation behavior are possible. This is valid not only for stable oxidation products but also for intermediates, as anal. can be carried out within a short time scale. Thus, a vast amount of valuable exptl. data can be acquired, which can help in understanding electrooxidation processes. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Electric Literature of 65-71-4
The Article related to electrooxidation thymine screen printed carbon electrode electrochem mass spectrometry, Electrochemistry: Electrochemical Cells and Systems and other aspects.Electric Literature of 65-71-4
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia