Abakir, Abdulkadir; Ruzov, Alexey published an article in 2021, the title of the article was Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry.Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione And the article contains the following content:
A review. Immunocytochem. can be instrumental in assessing the spatial distribution and relative levels of epigenetic modifications. Although conventional immunostaining has been utilized for the detection of 5-methylcytosine (5mC) in animal cells and tissues for several decades, the sensitivity of techniques based on the use of fluorophore-conjugated secondary antibodies is not always sufficient for studying DNA modifications that are less abundant in DNA compared with 5mC. Here we describe a protocol for sensitive immunocytochem. that utilizes peroxidase-conjugated secondary antibodies coupled with catalyzed reporter deposition and allows for detection of low-abundance noncanonical bases (e.g., 5-carboxylcytosine, 5caC, 5-formylcytosine, 5fC, 5-hydroxymethyluracil, 5hmU) in mammalian DNA. This method can be employed for evaluation of the levels and nuclear distribution of DNA modifications and permits their colocalization with protein markers in animal cells. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
The Article related to review dna modification signal amplification immunocytochem, 5-carboxylcytosine, 5-formylcytosine, dna (de)methylation, dna modifications, immunocytochemistry, immunofluorescence, immunohistochemistry, oxi-mcs, signal amplification and other aspects.Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia