Category: 1004524-64-4

Electric Literature of 1004524-64-4, With the rapid development and complex challenges of chemical substances, the synthesis of new drugs is usually one of the most effective ways to increase yield.1004524-64-4, name is 2-((4-(Aminomethyl)benzyl)oxy)pyrimidin-4-amine, molecular formula is C12H14N4O, molecular weight is 230.27, as common compound, the synthetic route is as follows.

Added to a 1.5 ml Eppendorf tube were 1 mumol of aminomethylbenzylcytosine in solution in 26 mul of anhydrous dimethylsulfoxide, 1 mumol of Tb(KR)-NHS in solution in 110 mul of anhydrous dimethylsulfoxide and 4 mul of diisopropylethylamine. The reaction mixture was stirred for two hours. The reaction was monitored by HPLC on a Merck Lichrospher RP 18, 5 mum, 125*4.6 column with a gradient of acetonitrile in water containing 25 mM of triethylammonium acetate. A purification by preparative HPLC was carried out on a Vydac C18, 10 mum, 250*22 column with a gradient of acetonitrile in water containing 25 mM of triethylammonium acetate. The fractions were collected and concentrated under reduced pressure. A white solid was obtained (350 nmol by optical density measurement, 35%) corresponding to the desired product. MS (ES+) m/z: [M+H+] 1645.2 [M+2H+]/2 823.4.

Statistics shows that 1004524-64-4 is playing an increasingly important role. we look forward to future research findings about 2-((4-(Aminomethyl)benzyl)oxy)pyrimidin-4-amine.

Reference:
Patent; Cis Bio International; Bourrier, Emmanuel; Laget, Michel; Lamarque, Laurent; Tinel, Norbert; Trinquet, Eric; Bazin, Herve; US9024022; (2015); B2;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Synthetic Route of 1004524-64-4, In the chemical reaction process,reaction time,type of solvent,can easily affect the result of the reaction, thereby determining the yield and properties of the reaction product.An updated downstream synthesis route of 1004524-64-4 as follows.

Example 3: N-[4-(4-Aminopyrimidin-2-yloymethyl)-benzyl]-tetramethylrhodamine-6-carboxamide (3) and N-[4-(4-Aminopyrimidin-2-yloxymethyl)-benzyl]-tetramethylrhodamine-5-carboxamide (4) [Show Image] 2-(4-(Aminomethyl)benzyloxy)pyrimidin-4-amine (2) (3.6 mg, 0.016 mmol) and 5(6)-carboxytetramethylrhodamine succinimidyl ester (8.2 mg, 0.016 mmol) are dissolved in 800 muL DMF with 2.4 muL TEA and heated overnight at 31 C. The solvent is evaporated in vacuo and the compounds are isolated by reversed phase MPLC (medium pressure liquid chromatography) on a C18 column using a linear gradient of water:acetonitrile (from 95:5 to 20:80 in 20 min, 0.08% TFA). MS (ESI) m/z 643 [M-CI]+.

These compound has a wide range of applications. It is believed that with the continuous development of the source of the synthetic route,1004524-64-4, its application will become more common.

Reference:
Patent; EPFL Ecole Polytechnique Federale de Lausanne; EP1882688; (2008); A1;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia