Category: 19030-75-2

Diaz, E. published the artcile2D NMR studies of the structures of the stereoselective adducts of the dehydrocostus lactone with pyrimidine derivatives, Formula: C7H10N2O2, the main research area is dehydrocostus lactone pyrimidine adduct NMR.

Anal. of 1H-1H n.O.e. effects observed for the adducts (I) (X = O, S; Y = O, NH2; Z = H, Me, F, Br, Pr, OMe), (II) and (III) on C-13 (10-18) of dehydrocostus lactone and different pyrimidine derivatives showed that H-7 and H-11 protons are in trans position. The NOESY cross peaks anal. and x-ray mol. structure of adduct I (X = Y =O, Z = H) are in excellent agreement with the exptl. data. Complete assignments of the 13C signals of some adducts based on 1D and 2D 1H and 13C NMR techniques are reported.

Spectroscopy Letters published new progress about NMR (nuclear magnetic resonance). 19030-75-2 belongs to class pyrimidines, name is 5-N-Propyluracil, and the molecular formula is C7H10N2O2, Formula: C7H10N2O2.

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Csarnyi, A. H. published the artcileSeparation of 5-alkyluracils and purine bases in hydrolyzates of enzymically synthesized nucleic acids by high-performance ion-pair liquid chromatography, Name: 5-N-Propyluracil, the main research area is DNA alkyluracil purine base chromatog; high performance ion pair chromatog alkyluracil.

Reversed-phase and reversed-phase ion-pair chromatog. methods were used to determine 5-alkyluracils in the presence of purine bases (mainly adenine) in the hydrolyzates of enzymically synthesized DNA. For the reversed-phase separations, a Hypersil ODS column was used with a 2-step gradient of 0.01M KH2PO4 (pH 5.5) in 80% MeOH. The eluate was monitored at 260 nm. For the ion-pair separations, octyl sulfate was the counter-ion and the solvent composition and pH varied. The effect of ionic strength, pH, and concentration of the ion-pairing agent and MeOH on the selectivity between alkyluracils and purine bases was examined in order to simplify the routine work and to reduce the time necessary for anal. Optimal conditions could be developed for the isocratic separation of the various mixtures obtained by hydrolysis of the products of enzymic synthesis.

Journal of Chromatography published new progress about DNA Role: BIOL (Biological Study). 19030-75-2 belongs to class pyrimidines, name is 5-N-Propyluracil, and the molecular formula is C7H10N2O2, Name: 5-N-Propyluracil.

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Hatano, Akihiko published the artcileOne-pot approach to functional nucleosides possessing a fluorescent group using nucleobase-exchange reaction by thymidine phosphorylase, COA of Formula: C7H10N2O2, the main research area is fluorescent nucleoside preparation nucleobase exchange reaction thymidine phosphorylase.

Herein, β-selective coupling is described between a modified uracil and a deoxyribose to produce functionalized nucleosides catalyzed by thymidine phosphorylase derived from Escherichia coli. This enzyme mediates nucleobase-exchange reactions to convert unnatural nucleosides possessing a large functional group such as a fluorescent mol., coumarin or pyrene, linked via an alkyl chain at the C5 position of uracil. 5-(Coumarin-7-oxyhex-5-yn)uracil (C4U) displayed 57.2% conversion at 40% DMSO concentration in 1.0 mM phosphate buffer pH 6.8 to transfer thymidine to an unnatural nucleoside with C4U as the base. In the case of using 5-(pyren-1-methyloxyhex-5-yn)uracil (P4U) as the substrate, TP also could catalyze the reaction to generate a product with a very large functional group at 50% DMSO concentration (21.6% conversion). Docking simulations were carried out using MF myPrest for the modified uracil bound to the active site of TP. The uracil moiety of the substrate binds to the active site of TP, with the fluorescent moiety linked to the C5 position of the nucleobase located outside the surface of the enzyme. As a consequence, the bulky fluorescent moiety binding to uracil has little influence on the coupling reaction.

Organic & Biomolecular Chemistry published new progress about Enzyme functional sites, active. 19030-75-2 belongs to class pyrimidines, name is 5-N-Propyluracil, and the molecular formula is C7H10N2O2, COA of Formula: C7H10N2O2.

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Votruba, Ivan published the artcileSynthesis of 2′-deoxy-β-D-ribonucleosides and 2,3-dideoxy-β-D-pentofuranosides on immobilized bacterial cells, Computed Properties of 19030-75-2, the main research area is deoxynucleoside transglycosylation immobilized Escherichia; nucleoside deoxy dideoxy biosynthesis Escherichia.

Alginate gel-entrapped cells of auxotrophic thymine-dependent strain of E. coli catalyze the transfer of 2-deoxy-D-ribofuranosyl moiety of 2′-deoxyuridine to purine and pyrimidine bases as well as their aza and deaza analogs. All experiments invariably gave β-anomers; in most cases, the reaction was regiospecific, affording N9-isomers in the purine and N1-isomers in the pyrimidine series. Also a 2,3-dideoxynucleoside can serve as donor of the glycosyl moiety. The acceptor activity of purine bases depends only little on substitution, the only condition being the presence of N7-nitrogen atom. On the other hand, in the pyrimidine series the activity is limited to only a narrow choice of mostly short 5-alkyl and 5-halo uracil derivatives Heterocyclic bases containing amino groups are deaminated; this can be avoided by conversion of the base to the corresponding N-dimethylaminomethylene derivative which is then ammonolyzed. The method was verified by isolation of 9-(2-deoxy-β-D-ribofuranosyl) derivatives of adenine, guanine, 2-chloroadenine, 6-methylpurine, 8-azaadenine, 8-azaguanine, 1-deazaadenine, 3-deazaadenine, 1-(2-deoxy-β-D-ribofuranosyl) derivatives of 5-ethyluracil, 5-fluorouracil, and 9-(2,3-dideoxy-β-D-pentofuranosyl)hypoxanthine, 9-(2,3-dideoxy-β-D-pentofuranosyl)-6-methylpurine, and other nucleosides.

Collection of Czechoslovak Chemical Communications published new progress about Escherichia coli. 19030-75-2 belongs to class pyrimidines, name is 5-N-Propyluracil, and the molecular formula is C7H10N2O2, Computed Properties of 19030-75-2.

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia