Category: 4270-27-3

Chemistry, like all the natural sciences, begins with the direct observation of nature¡ª in this case, of matter.4270-27-3, Name is 6-Chloropyrimidine-2,4(1H,3H)-dione, SMILES is O=C1NC(C=C(N1)Cl)=O, belongs to pyrimidines compound. In a document, author is Gary, Anne-Sophie, introduce the new discover, Name: 6-Chloropyrimidine-2,4(1H,3H)-dione.

Apoptosis, the only cell death pathway that can be measured in human diploid dermal fibroblasts following lethal UVB irradiation

Ultraviolet radiation (UVR) is a major environmental genotoxic agent. In skin, it can lead to the formation of mutagenic DNA damage. Several mechanisms are in place to prevent the conversion of these DNA damage into skin cancer-driver mutations. An important mutation prevention mechanism is the programmed cell death, which can safely dispose of the damaged cells. Apoptosis is the most studied and best characterised programmed cell death, but an increasing amount of new cell death pathways are emerging. Using different pharmacological cell death inhibitors and antioxidants, we have evaluated the implication of apoptosis, necroptosis, ferroptosis and parthanatos in UVB-induced cell death in human diploid dermal fibroblasts. Our results show that apoptosis is the only known cell death mechanism induced by UVB irradiation in fibroblasts. We also showed that lethal UVB irradiation induces a PARP-dependent drastic loss of cellular metabolic activity caused by an overused of NAD+.

The proportionality constant is the rate constant for the particular unimolecular reaction. the reaction rate is directly proportional to the concentration of the reactant. I hope my blog about 4270-27-3 is helpful to your research. Name: 6-Chloropyrimidine-2,4(1H,3H)-dione.

Reference:
Pyrimidine | C4H4N2 – PubChem,
,Pyrimidine – Wikipedia

Electric Literature of 4270-27-3, Catalysts allow a reaction to proceed via a pathway that has a lower activation energy than the uncatalyzed reaction. 4270-27-3, Name is 6-Chloropyrimidine-2,4(1H,3H)-dione, SMILES is O=C1NC(C=C(N1)Cl)=O, belongs to pyrimidines compound. In a article, author is Li, Yunlong, introduce new discover of the category.

Drug screening identified gemcitabine inhibiting hepatitis E virus by inducing interferon-like response via activation of STAT1 phosphorylation

Exposure to hepatitis E virus (HEV) bears a high risk of developing chronic infection in immunocompromised patients, including organ transplant recipients and cancer patients. We aim to identify effective anti-HEV therapies through screening and repurposing safe-in-human broad-spectrum antiviral agents. In this study, a safe-inhuman broad-spectrum antiviral drug library comprising of 94 agents was used. Upon screening, we identified gemcitabine, a widely used anti-cancer drug, as a potent inhibitor of HEV replication. The antiviral effect was confirmed in a range of cell culture models with genotype 1 and 3 HEV strains. As a cytidine analog, exogenous supplementation of pyrimidine nucleosides effectively reversed the antiviral activity of gemcitabine, but the level of pyrimidine nucleosides per se does not affect HEV replication. Surprisingly, similar to interferon-alpha (IFN alpha) treatment, gemcitabine activates STAT1 phosphorylation. This subsequently triggers activation of interferon-sensitive response element (ISRE) and transcription of interferon-stimulated genes (ISGs). Cytidine or uridine effectively inhibits gemcitabine-induced activation of ISRE and ISGs. As expected, JAK inhibitor 1 blocked IFN alpha, but not gemcitabine-induced STAT1 phosphorylation, ISRE/ISG activation, and anti-HEV activity. These effects of gemcitabine were completely lost in STAT1 knockout cells. In summary, gemcitabine potently inhibits HEV replication by triggering interferon-like response through STAT1 phosphorylation but independent of Janus kinases. This represents a non-canonical antiviral mechanism, which utilizes the innate defense machinery that is distinct from the classical interferon response. These results support repurposing gemcitabine for treating hepatitis E, especially for HEV-infected cancer patients, leading to dual anti-cancer and antiviral effects.

Electric Literature of 4270-27-3, Enzymes are biological catalysts that produce large increases in reaction rates and tend to be specific for certain reactants and products. I hope my blog about 4270-27-3 is helpful to your research.

Reference:
Pyrimidine | C4H4N2 – PubChem,
,Pyrimidine – Wikipedia

A catalyst don’t appear in the overall stoichiometry of the reaction it catalyzes, but it must appear in at least one of the elementary reactions in the mechanism for the catalyzed reaction. 4270-27-3, Name is 6-Chloropyrimidine-2,4(1H,3H)-dione, molecular formula is C4H3ClN2O2. In an article, author is Abdallah, M.,once mentioned of 4270-27-3, Application In Synthesis of 6-Chloropyrimidine-2,4(1H,3H)-dione.

Expired amoxicillin and cefuroxime drugs as efficient anticorrosives for Sabic iron in 1.0 M hydrochloric acid solution

The effects of two expired antibacterial drugs, amoxicillin (Amo) cefuroxime (Cef), on the corrosion behavior of Sabic iron in 1.0 M HCl solution were examined using weight loss, galvanostatic polarization (GAP), potentiodynamic anodic polarization, and electrochemical impedance spectroscopy techniques. The outcomes showed that the inhibition efficiency increased with increasing concentrations of Amo and Cef and decreased with temperature. The activity of inhibition of these compounds was elucidated by adsorption on Sabic iron surfaces. The adsorption process obeyed the Langmuir isotherm. The activation and adsorption thermodynamic parameters have been determined and clarified. GAP studies indicated that expired Amo and Cef served as mixed inhibitors. The impedance data showed capacitive loop which indicates that charge transfer governs corrosion reactions. Expired Amo and Cef drugs are good pitting inhibitors by positively shifting the pitting potential. There is a complete agreement between the inhibition efficacies obtained from the different measurements

Interested yet? Keep reading other articles of 4270-27-3, you can contact me at any time and look forward to more communication. Application In Synthesis of 6-Chloropyrimidine-2,4(1H,3H)-dione.

Reference:
Pyrimidine | C4H4N2 – PubChem,
,Pyrimidine – Wikipedia

Related Products of 4270-27-3, As we all know, there are many different methods for the synthesis of a compound, and people can choose the synthesis method that suits their own laboratory according to the actual situation. 4270-27-3, name is 6-Chloropyrimidine-2,4(1H,3H)-dione, molecular formula is C4H3ClN2O2, The compound is widely used in many fields, so it is necessary to find a new synthetic route. The downstream synthesis method of this compound is introduced below.

General procedure: To a suspension of 6-chlorouracil (17.58 g, 120 mmol) in DMF (60 mL) were added DIPEA (27.2 mL, 156 mmol) and 1-bromo-2-butyne (12 mL, 132 mmol). The reaction mixture was stirred overnight at r.t. Water was added. The precipitate was collected by filtration, washed with water and EtOH, and dried to give 5b as a light yellow solid (21 g, 88%).

According to the analysis of related databases, 4270-27-3, the application of this compound in the production field has become more and more popular.

Reference:
Article; Lai, Zeng-Wei; Li, Chunhong; Liu, Jun; Kong, Lingyi; Wen, Xiaoan; Sun, Hongbin; European Journal of Medicinal Chemistry; vol. 83; (2014); p. 547 – 560;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Synthetic Route of 4270-27-3, Adding some certain compound to certain chemical reactions, such as: 4270-27-3, name is 6-Chloropyrimidine-2,4(1H,3H)-dione,molecular formula is C4H3ClN2O2, can increase the reaction rate and produce products with better performance than those obtained under traditional synthetic methods. Here is a downstream synthesis route of the compound 4270-27-3.

6-chloro-uracil (7 g, 1 eq.), methyl iodide (8.9 ml, 3 eq.) and anhydrous potassium carbonate (3.36 g, 0.5 eq.) were stirred in 30 ml of dimethylsulfoxide at room temperature for 3 h, then 38 ml of water was added and stirred in ice bath for 2 h. A white solid precipitated and the precipitate was collected by filtration and dried to give 5.12 g of a solid. MS (ESI): 161(M+H)

According to the analysis of related databases, 4270-27-3, the application of this compound in the production field has become more and more popular.

Reference:
Patent; Shanghai Institute Of Materia Medica Chinese Academy of Sciences; SHEN, Jianhua; WANG, Yiping; CHEN, Xinde; XU, Wenwei; WANG, Kai; (84 pag.)EP3239135; (2017); A1;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Adding a certain compound to certain chemical reactions, such as: 4270-27-3, 6-Chloropyrimidine-2,4(1H,3H)-dione, can increase the reaction rate and produce products with better performance than those obtained under traditional synthetic methods. Here is a downstream synthesis route of the compound, 4270-27-3, blongs to pyrimidines compound. Recommanded Product: 6-Chloropyrimidine-2,4(1H,3H)-dione

Reference Example 17. 6-Chloro-1-(2-trimethylsilyl)ethoxymethylpyrimidine-2,4-dione In a similar manner to the procedures described in Reference Example 3, reactions were carried out using 6-chloropyrimidine-2,4-dione, instead of pyrimidine-2,4-dione, and using 2-(trimethylsilyl)ethoxymethyl chloride, instead of benzyloxymethyl chloride, to give the title compound (yield 71%) as a white powder. 1H-Nuclear magnetic resonance spectrum (400 MHz, CDCl3) delta ppm: 9.10 (1H, br.s), 5.93 (1H, s), 5.45 (2H, s), 3.68 (2H, t, J=8Hz), 0.96 (2H, t, J=8Hz), 0.01 (9H, s).

These compound has a wide range of applications. It is believed that with the continuous development of the source of the synthetic route,4270-27-3, its application will become more common.

Reference:
Patent; Sankyo Company Limited; EP1069110; (2001); A1;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Application of 4270-27-3, Adding some certain compound to certain chemical reactions, such as: 4270-27-3, name is 6-Chloropyrimidine-2,4(1H,3H)-dione,molecular formula is C4H3ClN2O2, can increase the reaction rate and produce products with better performance than those obtained under traditional synthetic methods. Here is a downstream synthesis route of the compound 4270-27-3.

Step – I: Preparation of 2-((6-chloro-2,4-dioxo-3,4-dihydropyrimidin-l(2H)- yl)methyl)benzonitrileTo a 6-chloropyrimidine-2,4(l H,3H)-dione (21 gm) was added N- methylpyrrolidone (80 ml) under stirring and then added diisopropylethylamine (17 ml) slowly for 30 minutes. To the solution was added a solution of 2- (bromomethyl)benzonitrile (20 gm) in toluene (80 ml) slowly for 1 hour and the temperature of the reaction mass was raised to 70 to 80C. The reaction mass was maintained for 4 hours at 70 to 80C and then cooled to 15 to 20C. The reaction mass was quenched with water and maintained for 30 minutes at 15 to 20C. The reaction mass was then cooled to 0 to 5C and stirred for 1 hour. The reaction mass was filtered and washed with water and hexane to obtain a wet solid. To the wet solid thus obtained was added water (200 ml) and pH was adjusted to 9.0 to 10.0 with sodium hydroxide (IN). The reaction mass was stirred for 30 minutes and then added methylene chloride (300 ml) and then the layers were separated. The pH of the aqueous layer was adjusted to 4.0 to 5.0 with hydrochloric acid (IN) and stirred for 30 minutes. The solid obtained was collected by filtration and then dried to obtain 19.3 gm of 2-((6-chloro-2,4-dioxo-3,4- dihydropyrimidin-l(2H)-yl)methyl)benzonitrile.

According to the analysis of related databases, 4270-27-3, the application of this compound in the production field has become more and more popular.

Reference:
Patent; HETERO RESEARCH FOUNDATION; PARTHASARADHI REDDY, Bandi; RATHNAKAR REDDY, Kura; MURALIDHARA REDDY, Dasari; MOHANBABU, Maradolla; VAMSI KRISHNA, Bandi; WO2013/46229; (2013); A1;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Adding a certain compound to certain chemical reactions, such as: 4270-27-3, 6-Chloropyrimidine-2,4(1H,3H)-dione, can increase the reaction rate and produce products with better performance than those obtained under traditional synthetic methods. Here is a downstream synthesis route of the compound, Recommanded Product: 6-Chloropyrimidine-2,4(1H,3H)-dione, blongs to pyrimidines compound. Recommanded Product: 6-Chloropyrimidine-2,4(1H,3H)-dione

4-Dimethylamino-l-aminoindan (8.5g, 48.2 ?unol) and 6- chlorouracil (3.55g, 24.2 mmol) were dissolved in DMSO (8.5 ml) , heated to 1150C and stirred for 4h. The reaction mixture was cooled to 800C and glyme (ethylene glycol dimethylether) was added to provide a thick mixture which was further refluxed for Ih. The mixture was cooled to room temperature, and the solid collected by filtration and washed well with glyme. The white solid was treated with water (50 ml) and the suspension was refluxed for 30 min, cooled to room temperature, filtered and washed thoroughly with Et2O. A white solid (4g, 58%) was provided. The free base was converted to the HCl salt by dissolving it in EtOH (32 ml) and HCl/EtOH (28% solution, 2.5 ml) and adding Et2O (70 ml) . The salt was ; cdeltalectec TSy rl”‘tfrat&n, washealpha8 with Et2O and dried to give an off-white powder (4.8g, 58%). Mp 192-193C. 1H-NMR (free base) DMSO-d6 delta :10.24 (br epsilon, IH, CONHCO), 9.68 (br s, IH, CONHC), 7.15 (br t, IH, J = 8 Hz, Ar), 6.85 and 6.78 (two br d, 2H, Ar), 6.42 (br d, IH, J = 6 Hz , CHNH), 4.87 (br q, IH, J = 7 Hz , CHNH), 4.64 (br s, IH, CCHCO), 2.75 -2.90 (br s & br m, 8H, Me2N S- CH2CH2C), 2.45 and 1.76 (m, 2H, CHCH2); 13C (free base) DMS0-ds delta : 164.27 (CHCO), 153.70 (NHCNH), 150.72 (NHCONH), 149.80 (Me2NC), 144.03, 133.59, 127.61, 116.0, 115.39, 73.27 (CHCO), 56.64 (CHNH), 42.37 (Me2N), 33.21, 29.41; Anal, (calcd for Ci5Hi8N4O2) C 62.92, H 6.34, N 19.57. Found C 62.73, H 6.45, N 19.20; MS (CI) (iBu) m/z (286.11, M); HRMS (CI, iBu) exact mass calcd for Ci5H18N4O2 286.1429, found 286.1450. 1H-NMR (HCl salt) DMS0-d6 delta: 10.38 (br s, IH, CONHCO), 10.27 (br s, IH, CONHC), 7.64 (br d, IH, J = 8 Hz, Ar), 7.45 and 7.39 (br t & br d, 2H, Ar), 7.20 (br d, IH, J = 6 Hz, CHNH), 5.00 (br q, IH, J = 7 Hz, CHNH), 4.74 (br s, IH, CCHCO), 3.35 (br m, IH, CH2CH2C), 3.0-3.12 (br s & m, 7H, Me2N & CH2CH2C), 2.57 and 1.85 (two m, 2H, CHCH2); 13C (HCl salt) DMSO-d6 delta : 164.26 (CHCO), 154.13 (NHCNH), 150.37 (Me2NC), 145.90 (NHCONH), 140.07, 135.55, 128.65, 124.39, 119.64, 73.09 (CHCO), 56.39 (CHNH), 44.78 (Me2N), 32.87, 28.29; MS (CI) (NH3) m/z (287, MH+) .

At the same time, in my other blogs, there are other synthetic methods of this type of compound,4270-27-3, 6-Chloropyrimidine-2,4(1H,3H)-dione, and friends who are interested can also refer to it.

Reference:
Patent; TEVA PHARMACEUTICAL INDUSTRIES, LTD.; TEVA PHARMACEUTICALS USA, INC.; WO2006/20070; (2006); A2;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Related Products of 4270-27-3 , The common heterocyclic compound, 4270-27-3, name is 6-Chloropyrimidine-2,4(1H,3H)-dione, molecular formula is C4H3ClN2O2, its traditional synthetic route has been very mature, but the traditional synthetic route has various shortcomings, such as complicated route, low yield, poor purity, etc., below Introduce a new synthetic route.

[Example 31] Preparation of 1-(4-chlorobenzyl)-3-(2-methoxycarbonyl-2-methylpropyl)-6-(6-trifluoromethyl-2-pyridylamino)pyrimidine-2,4(1H, 3H)-dion (I-108) To a mixture of 6-chlorouracil (5.00 g, 34.1 mmol) and DMF (100 mL) were added 60% sodium hydride (1.64 g, 40.9 mmol) and lithium bromide (2.96 g, 34.18 mmol), and the resulting mixture was stirred at room temperature for 1 hour. To the reaction mixture was added 4-chlorobenzylbromide (7.71 g, 37.5 mmol), and the resulting mixture was stirred for additional 21 hours. To the reaction mixture was added water (100 mL), and the precipitated solid was filtered off. The solid was purified by silica gel column chromatography (ethyl acetate/hexane) and precipitated by methanol/ethyl acetate/hexane to give 6-chloro-1-(4-chlorobenzyl)pyrimidine-2,4(1H, 3H)-dion (3.87 g, Yield: 42%) as colorless solid. 1H-NMR (delta ppm TMS/DMSO-d6): 5.14 (2H, s), 5.96 (1H, s), 7.31 (2H, d, J=8.0 Hz), 7.43 (2H, d, J=8.0 Hz), 11.76 (1H, s).

The synthetic route of 4270-27-3 has been constantly updated, and we look forward to future research findings.

Reference:
Patent; Shionogi & Co., Ltd.; KAI, Hiroyuki; ENDOH, Takeshi; JIKIHARA, Sae; ASAHI, Kentaro; HORIGUCHI, Tohru; EP2604260; (2013); A1;,
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia