Category: 771-81-3

In 2006,Plant disease included an article by Luan, Y S; Feng, L; An, L J. Formula: C6H7N3O2S. The article was titled 《First Report of Blueberry Leaf Spot Caused by Cylindrocladium colhounii in China.》. The information in the text is summarized as follows:

During late July and early August of 2005, leaf spot symptoms were observed in a blueberry nursery at a plantation in Dalian, which to our knowledge, lies within the largest blueberry-production area in China. Symptoms were observed primarily on lowbush species, for example Blomidon, as well as half-highbush cultivars. A slow-growing, white mycelium from the margin of necrotic leaf spots was recovered on potato dextrose agar (PDA). The following morphological traits were observed: erect conidiophores that branch twice and were terminated in a stiped, clavate phialide; hyaline, cylindrical, four-celled conidia; and globose, reddish brown, aggregated chlamydospores. Conidiophores (including stipes and terminal phialides) were 305 to 420 × 5 to 9 μm; primary branches were 9 to 45 × 5 to 6.3 μm; secondary branches were 9 to 17.3 × 3.1 to 4.5 μm; phialides were 7.8 to 17.5 × 2.5 to 6 μm; stipes (from the highest branch area to vesicle) were 150 to 270 μm long; and vesicles were 13 to 30 × 2 to 4.5 μm. Conidia were 50 to 72 × 4 to 5.5 μm. Chlamydospores were 15 to 20 μm in diameter. Koch’s postulates were fulfilled by spray inoculating two healthy cultivars with conidiophores homogenized in axenic water. As a control, two healthy plants were sprayed with axenic water. Plants were placed inside plastic bags to maintain humidity and incubated in a growth chamber at 26°C under fluorescent light for 14 h and 20°C in darkness for 10 h. After 2 days, the plastic bags were removed and plants were maintained under the same conditions. After 4 days, small-to-medium brown spots with purplish margins were observed on the adaxial side of leaves from inoculated plants, but not from control plants. Fungi isolated from these lesions had the same morphological traits as the ones isolated previously from field plants. The morphological descriptions and measurements were similar to Cylindorocladium colhounii (2). The 5.8S subunit and flanking internal transcribed spacers (ITS1 and ITS2) of rDNA and the β-tubulin gene were amplified from DNA extracted from single-spore cultures using the ITS1/ITS4 primers and T1/Bt2b primers, respectively, and sequenced (1). The ITS and β-tubulin gene sequences were similar to C. colhounii STE-U 1237 (99%; GenBank Accession Number AF231953) and C. colhounii STE-U 705 (99%; GenBank Accession Number AF231954), respectively. The morphology, secondary conidiation, and sequences of ITS and β-tubulin gene identify the causal fungus as C. colhounii. To our knowledge, this is the first report of C. colhounii on blueberry in China or in the world. References: (1) P. W. Crous et al. Can. J. Bot. 77:1813, 1999. (2) T. Watanabe. Page 222 in: Dictorial Atlas of Soil and Seed Fungi. CRC Press, Inc., Boca Raton, Fl, 1994. In the part of experimental materials, we found many familiar compounds, such as 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3Formula: C6H7N3O2S)

4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3) belongs to anime.Typically the presence of an amine functional group is deduced by a combination of techniques, including mass spectrometry as well as NMR and IR spectroscopies. 1H NMR signals for amines disappear upon treatment of the sample with D2O. In their infrared spectrum primary amines exhibit two N-H bands, whereas secondary amines exhibit only one.Formula: C6H7N3O2S

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

In 2012,Plant disease included an article by Pan, R; Deng, Q; Xu, D; Ji, C; Deng, M; Chen, W. Recommanded Product: 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid. The article was titled 《First Report of Peanut Cylindrocladium Black Rot Caused by Cylindrocladium parasiticum in Fujian Province, Eastern China.》. The information in the text is summarized as follows:

During late July and early August of 2010, a serious disease of peanut (Arachis hypogaea L.) resembling Cylindrocladium black rot (CBR) was found in Longyan City, Fujian Province of Eastern China. Aboveground symptoms were chlorosis and yellowing of leaves, a black rot of the basal stem and pegs, and wilting of the vines. Underground plant parts (including pods, pegs, taproots, and hypocotyls) were blackened and rotted. Orange-to-reddish spherical fruiting bodies appeared on the lesions of the basal stems and pegs of peanut. Disease incidence was approximately 20%. A fungus was consistently isolated from the edge of lesions on potato dextrose agar (PDA) amended with streptomycin and incubated at 25°C. The fungus produced white-to-pale buff mycelia with a yellowish brown pigment. Optimum growth of the fungus on PDA was at 25 to 30°C. Conidiophores were borne laterally on a stipe that terminated in a hyaline, globose vesicle measuring 5.5 × 10.9 μm in diameter. Conidia were hyaline, cylindrical, rounded at both ends, slightly wider at the base than at the apex, with one to three septa (mostly one septa), and measured 27.3 to 70.9 × 4.1 to 8.2 μm. Orange-to-reddish perithecia were readily formed in old cultures. The perithecia were subglobose to oval or obovate and measured 215.6 to 609.4 × 309.4 to 496.9 μm. The asci were hyaline, clavate, thin walled, long stalked, with each containing eight ascospores. Ascospores were hyaline, falcate, had one septum, and measured 27.3 to 54.5 × 4.1 to 6.8 μm. The fungus was identified as Cylindrocladium parasiticum Crous, M.J. Wingfield, & Alfenas (teleomorph Calonectria ilicicola) (1,2). The beta-tubulin gene fragment was amplified using the T1/Bt2b primers (3) and sequenced. The sequences of three isolates (GenBank Accession Numbers JF343965, JF429656, and JF429657), when compared with existing sequences in GenBank, had 95 to 99% sequence identity with Calonectria ilicicola (GenBank Accession Numbers AY725643 and AY725639). Pathogenicity tests were conducted by first culturing the fungus on wheat kernels for 2 weeks. Inoculated kernels were then used as inoculum and mixed with sterilized soil in a proportion of 1:20 by weight in plastic pots (10 × 9 cm). Noninoculated wheat kernels were mixed with sterilized soil in the same proportion and served as the control. Two-week-old peanut seedlings (cv. Yueyou Number 7) were transplanted into inoculated or noninoculated pots. There were five plants per pot and each treatment was replicated four times. The plants were incubated in a greenhouse at 25 ± 2°C. All of the treated plants exhibited typical basal stem and root rot symptoms of CBR 2 weeks after inoculation, while all of the control plants remained healthy. C. parasiticum was reisolated from the diseased plants. To our knowledge, this is the first report of CBR on peanut in Fujian Province in Eastern China. The disease has been previously reported in Guangdong Province in Southern China but is not known elsewhere (4). This pathogen may pose a serious threat to peanut production in China, where peanut is an important crop. References: (1) D. K. Bell and E. K. Sobers. Phytopathology 56:1361, 1966. (2) P. W. Crous et al. Mycol. Res. 97:889, 1993. (3) P. W. Crous et al. Can. J. Bot. 77:1813, 1999. (4) R. Pan et al. Plant Pathol. 58:1176, 2009. In the experiment, the researchers used many compounds, for example, 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3Recommanded Product: 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid)

4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3) belongs to anime. To avoid the problem of multiple alkylation, methods have been devised for “blocking” substitution so that only one alkyl group is introduced. The Gabriel synthesis is one such method; it utilizes phthalimide, C6H4(CO)2NH, whose one acidic hydrogen atom has been removed upon the addition of a base such as KOH to form a salt.Recommanded Product: 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

HPLC of Formula: 771-81-3On November 24, 1998 ,《The C-Terminal Region of the Stalk Domain of Ubiquitous Human Kinesin Heavy Chain Contains the Binding Site for Kinesin Light Chain》 appeared in Biochemistry. The author of the article were Diefenbach, Russell J.; Mackay, Joel P.; Armati, Patricia J.; Cunningham, Anthony L.. The article conveys some information:

The motor protein kinesin is a heterotetramer composed of two heavy chains of ∼120 kDa and two light chains of ∼65 kDa protein. Kinesin motor activity is dependent on the presence of ATP and microtubules. The kinesin light chain-binding site in human kinesin heavy chain was determined by reconstituting in vitro a complex of recombinant heavy and light chains. The proteins expressed in bacteria included oligohistidine-tagged fragments of human ubiquitous kinesin heavy chain, spanning most of the stalk and all of the tail domain (amino acids 555-963); and untagged, essentially full-length human kinesin light chain (4-569) along with N-terminal (4-363) and C-terminal (364-569) light chain fragments. Heavy chain fragments were attached to Ni2+-charged beads and incubated with untagged light chain fragments. Anal. of eluted complexes by SDS-PAGE and immunoblotting mapped the light chain-binding site in heavy chain to amino acids 771-813, a region close to the C-terminal end of the heavy chain stalk domain. In addition, only the full-length and N-terminal kinesin light chain fragments bound to this heavy chain region. Within this heavy chain region are four highly conserved contiguous heptad repeats (775-802) which are predicted to form a tight α-helical coiled-coil interaction with the heptad repeat-containing N-terminus of the light chain, in particular region 106-152 of human light chain. This predicted hydrophobic, α-helical coiled-coil interaction is supported by both CD spectroscopy of the recombinant kinesin heavy chain fragment 771-963, which displays an α-helical content of 70%, and the resistance of the heavy/light chain interaction to high salt (0.5 M). After reading the article, we found that the author used 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3HPLC of Formula: 771-81-3)

4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3) belongs to anime. Nitrous acid converts secondary amines (aliphatic or aromatic) to N-nitroso compounds (nitrosamines): R2NH + HNO2 → R2N―NO. Some nitrosamines are potent cancer-inducing substances, and their possible formation is a serious consideration when nitrites, which are salts of nitrous acid, are present in foods or pharmaceutical preparations. Tertiary amines give rise to nitrosamines more slowly; an alkyl group is eliminated as an aldehyde or ketone, along with nitrous oxide, N2O.HPLC of Formula: 771-81-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

《Pyridopyrimidinone derivatives as potent and selective c-Jun N-terminal kinase (JNK) inhibitors》 was written by Zheng, Ke; Park, Chul Min; Iqbal, Sarah; Hernandez, Pamela; Park, HaJeung; LoGrasso, Philip V.; Feng, Yangbo. Computed Properties of C6H7N3O2S And the article was included in ACS Medicinal Chemistry Letters on April 9 ,2015. The article conveys some information:

A novel series of 2-aminopyridopyrimidinone based JNK (c-jun N-terminal kinase) inhibitors were discovered and developed. Structure-activity relationships (SARs) were systematically developed utilizing biochem. and cell based assays and in vitro and in vivo drug metabolism and pharmacokinetic (DMPK) studies. Through the optimization of lead compound 1, several potent and selective JNK inhibitors with high oral bioavailability were developed. (trans)-1-Isopropyl-3-[4-(8-isopropyl-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidin-2-yl-amino)-cyclohexyl]urea (I), was a potent JNK3 inhibitor (IC50 = 15 nM), had high selectivity against p38 (IC50 > 10 μM), had high potency in functional cell based assays, and had high stability in human liver microsome (t1/2 = 76 min), a clean CYP-450 inhibition profile, and excellent oral bioavailability (%F = 87). Moreover, cocrystal structures of (I) and (trans)-1-[4-(8-Cyclopentyl-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidin-2-ylamino)-cyclohexyl]-3-isopropyl-urea in JNK3 were solved at 2.0 Å. These structures elucidated the binding mode (Type-I binding) and can pave the way for further inhibitor design of this pyridopyrimidinone scaffold for JNK inhibition. The results came from multiple reactions, including the reaction of 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3Computed Properties of C6H7N3O2S)

4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3) belongs to anime. Amines can be classified according to the nature and number of substituents on nitrogen. Aliphatic amines contain only H and alkyl substituents. Aromatic amines have the nitrogen atom connected to an aromatic ring.Important amines include amino acids, biogenic amines, trimethylamine, and aniline. Inorganic derivatives of ammonia are also called amines, such as monochloramine (NClH2).Computed Properties of C6H7N3O2S

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

COA of Formula: C6H7N3O2SOn March 1, 2005, Du, Miao; Jiang, Xiu-Juan; Zhao, Xiao-Jun published an article in Acta Crystallographica, Section E: Structure Reports Online. The article was 《Diaquabis(2,5-di-3-pyridyl-1,3,4-oxadiazole)dithiocyanatomanganese(II): a three-dimensional supramolecular network formed through O-H···N and C-H···S interactions》. The article mentions the following:

Crystals of the neutral mononuclear title complex are triclinic, space group P1̅, with a 8.1951(19), b 8.762(2), c 10.619(3) Å, α 82.472(3), β 77.181(3), γ 79.873(3)°; Z = 1, dc = 1.494; R = 0.035, w(F2) = 0.099 for 2540 reflections. The structure is centrosym.; the MnII atom lies on an inversion center and is six-coordinate (MnN4O2), with an octahedral geometry comprising two trans monodentate 2,5-di-3-pyridyl-1,3,4-oxadiazole ligands, two thiocyanate ligands and two bound H2O mols. Intermol. O-H···N H bonds between these monomeric units result in two-dimensional supramol. layers with a parallel arrangement, which are stabilized by intralayer aromatic stacking and further extended to a three-dimensional network via interlayer weak C-H···S interactions. The results came from multiple reactions, including the reaction of 4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3COA of Formula: C6H7N3O2S)

4-Amino-2-(methylthio)pyrimidine-5-carboxylic acid(cas: 771-81-3) belongs to anime. Left-handed and right-handed forms (mirror-image configurations, known as optical isomers or enantiomers) are possible when all the substituents on the central nitrogen atom are different (i.e., the nitrogen is chiral). With amines, there is extremely rapid inversion in which the two configurations are interconverted.COA of Formula: C6H7N3O2S

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia