On October 1, 2020, Cheng, Yuanlei; Zhang, Yashuo; Gong, Zhou; Zhang, Xinghua; Li, Yutong; Shi, Xiangqian; Pei, Yufeng; You, Huijuan published an article.COA of Formula: C5H6N2O2 The title of the article was High Mechanical Stability and Slow Unfolding Rates Are Prevalent in Parallel-Stranded DNA G-Quadruplexes. And the article contained the following:
Guanine-rich repeat sequences are known to adopt diverse G-quadruplex (G4) topologies. Determining the unfolding rates of individual G4 species is challenging due to the coexistence of multiple G4 conformations in a solution Here, using single-mol. magnetic tweezers, we systematically measured the unfolding force distributions of 4 oncogene promoter G4s, 12 model sequences with two 1-nucleotide (nt) thymine loops that predominantly adopt parallel-stranded G4 structures, and 6 sequences forming multiple G4 structures. All parallel-stranded G4s reveal an unfolding force peak at 40-60 pN, which is associated with extremely slow unfolding rates on the order of 10-5-10-7 s-1. In contrast, nonparallel G4s and partially folded intermediate states reveal an unfolding force peak <40 pN. These results suggest a strong correlation between the parallel-stranded G4s folding topol. and the slow unfolding rates and provide important insights into the mechanism that govern the stability and the transition kinetics of G4s. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).COA of Formula: C5H6N2O2
The Article related to parallel stranded dna g quadruplex unfolding kinetics mech stability, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.COA of Formula: C5H6N2O2
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia