Csarnyi, A. H. published the artcileSeparation of 5-alkyluracils and purine bases in hydrolyzates of enzymically synthesized nucleic acids by high-performance ion-pair liquid chromatography, Name: 5-N-Propyluracil, the main research area is DNA alkyluracil purine base chromatog; high performance ion pair chromatog alkyluracil.
Reversed-phase and reversed-phase ion-pair chromatog. methods were used to determine 5-alkyluracils in the presence of purine bases (mainly adenine) in the hydrolyzates of enzymically synthesized DNA. For the reversed-phase separations, a Hypersil ODS column was used with a 2-step gradient of 0.01M KH2PO4 (pH 5.5) in 80% MeOH. The eluate was monitored at 260 nm. For the ion-pair separations, octyl sulfate was the counter-ion and the solvent composition and pH varied. The effect of ionic strength, pH, and concentration of the ion-pairing agent and MeOH on the selectivity between alkyluracils and purine bases was examined in order to simplify the routine work and to reduce the time necessary for anal. Optimal conditions could be developed for the isocratic separation of the various mixtures obtained by hydrolysis of the products of enzymic synthesis.
Journal of Chromatography published new progress about DNA Role: BIOL (Biological Study). 19030-75-2 belongs to class pyrimidines, name is 5-N-Propyluracil, and the molecular formula is C7H10N2O2, Name: 5-N-Propyluracil.
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia