del Arco, Jon; Acosta, Javier; Pereira, Humberto M.; Perona, Almudena; Lokanath, Neratur K.; Kunishima, Naoki; Fernandez-Lucas, Jesus published an article in 2018, the title of the article was Enzymatic Production of Non-Natural Nucleoside-5′-Monophosphates by a Thermostable Uracil Phosphoribosyltransferase.Formula: C5H6N2O2 And the article contains the following content:
The use of enzymes as biocatalysts applied to synthesis of modified nucleoside-5′-monophosphates (NMPs) is an interesting alternative to traditional multistep chem. methods which offers several advantages, such as stereo-, regio-, and enantioselectivity, simple downstream processing, and mild reaction conditions. Herein we report the recombinant expression, production, and purification of uracil phosphoribosyltransferase from Thermus themophilus HB8 (TtUPRT). The structure of TtUPRT has been determined by protein crystallog., and its substrate specificity and biochem. characteristics have been analyzed, providing new structural insights into the substrate-binding mode. Biochem. characterization of the recombinant protein indicates that the enzyme is a homotetramer, with activity and stability across a broad range of temperatures (50-80 °C), pH (5.5-9) and ionic strength (0-500 mM NaCl). Surprisingly, TtUPRT is able to recognize several 5 and 6-substituted pyrimidines as substrates. These exptl. results suggest TtUPRT could be a valuable biocatalyst for the synthesis of modified NMPs. The experimental process involved the reaction of 6-Methylpyrimidine-2,4(1H,3H)-dione(cas: 626-48-2).Formula: C5H6N2O2
The Article related to uracil phosphoribosyltransferase nucleoside monophosphate, Fermentation and Bioindustrial Chemistry: Pharmaceuticals (Including Nutrients) and other aspects.Formula: C5H6N2O2
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia