The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Synthesis and anticancer activity of heteroaromatic linked 4β-amido podophyllotoxins as apoptotic inducing agents》. Authors are Kamal, Ahmed; Tamboli, Jaki R.; Vishnuvardhan, M. V. P. S.; Adil, S. F.; Nayak, V. Lakshma; Ramakrishna, S..The article about the compound:5-(3,4-Dichlorophenyl)-1H-pyrazole-3-carboxylic acidcas:276684-04-9,SMILESS:O=C(C1=NNC(C2=CC=C(Cl)C(Cl)=C2)=C1)O).COA of Formula: C10H6Cl2N2O2. Through the article, more information about this compound (cas:276684-04-9) is conveyed.
A series of different heteroaromatic linked 4β-amidopodophyllotoxin conjugates were synthesized and evaluated for anticancer activity against five human cancer cell lines. Among the series, one of the compound I showed significant antiproliferative activity in A549 (lung cancer) cell line. Flow cytometric anal. showed that I arrested the cell cycle in the G2/M phase leading to caspase-3 dependent apoptotic cell death. Further, Hoechst 33258 staining and DNA fragmentation assay also suggests that I induces cell death by apoptosis.
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Reference:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia