Normal-phase liquid chromatography coupled with electrospray ionization mass spectrometry for chiral separation and quantification of clevudine and its enantiomer in human plasma was written by Ding, Cungang; Ge, Qinghua; Wang, Yemu; Zhou, Zhen; Zhi, Xiaojin; Liu, Xiaofen; Li, Zhou. And the article was included in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences in 2012.Formula: C10H13FN2O5 The following contents are mentioned in the article:
A new, simple, and enantioselective normal-phase liquid chromatog.-mass spectrometry method was presented for the quantification of clevudine and its enantiomer in human blood plasma. A C18 cartridge was used in this method to extract the enantiomers in 200 μL plasma followed by a chiral separation on a cellulose-based LC column with mobile phase consisted of hexane, MeOH, and EtOH (62:28:10, V/V/V). The eluate was directed to a mass spectrometry through an electrospray ionization interface. A transition of m/z 261.0 to m/z 126.8 was used for monitoring of clevudine and its enantiomer. This method showed good linearity (R > 0.997), precision (<9.6%), and accuracy (within 95.48-105.9%) within a range of 10-1000 ng/mL for the enantiomers and was applied to the pharmacokinetics study of clevudine capsules in human plasma. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Formula: C10H13FN2O5).
1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. Heterocyclic compounds bearing the pyrimidine core are of tremendous interest as they constitute an important class of natural and synthetic compounds exhibiting diverse useful biological activities that hold attractive potential for clinical translation as therapeutic agents in alleviation of a myriad of diseases. Pyrimidine derivatives have been used in a wide variety of pharmaceuticals including general anesthetics, anti-epilepsy medication, anti-malaria medication, drugs for treating high blood pressure, and HIV medication.Formula: C10H13FN2O5
69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3