Development and validation of stability indicating HPLC method for the estimation of Minoxidil and related substance in topical formulation was written by Gaidhane, Hemant K.; Bidada, Jagdish P.; Bhusari, Akshay S.; Navkhare, Manjusha S.; Diwanka, Ganesh P.; Tiwari, Ashish H.. And the article was included in Journal of Pharmacy Research on December 31,2011.SDS of cas: 35139-67-4 The following contents are mentioned in the article:
A sensitive HPLC method was developed and validated for the determination of minoxidil and related substances in a novel topical formulation. The highly polar mol. requires aqueous mobile phase for the elution and separation of Minoxidil and its impurities (Impurity A, B and E). The developed method is found to be specific, reproducible and stability indicating. The Phenomenex Synergi Polar RP 250 × 4.6 mm 4μ column was used and mobile phase contains 2.0mL of di-Et amine and 40mL of glacial acetic acid in 1000mL of milli Q water (Buffer) and 20% THF in methanol (mobile phase A & B resp.) to achieve good resolution and retention of the analyte and its related impurities. The detector linearity was established from concentrations ranging from 0.02-400 μg/mL for Minoxidil and from 0.02-1.0 μg/mL for related substances with a correlation co-efficient of 0.999. The relative response factor (RRF) values of impurity A, impurity B and impurity E determined from linearity plots were 0.24, 0.31 and 1.07 resp. The limit of detection (LOD) and limit of quantification (LOQ) found to be 0.02μg/mL and 0.1μg/mL resp. for Minoxidil and related substances. The mol. was stable in all the stress conditions such as acid, base, heat photolysis and unstable in oxidation as per the recommendations of ICH guidelines. The method was proved to be robust with respect to changes in flow rate, pH and column temperature This study involved multiple reactions and reactants, such as 2,6-Diamino-4-chloropyrimidine-1-oxide (cas: 35139-67-4SDS of cas: 35139-67-4).
2,6-Diamino-4-chloropyrimidine-1-oxide (cas: 35139-67-4) belongs to pyrimidine derivatives. The pyrimidine derivatives can easily interact with enzymes, genetic materials, and bio components within the cell. For example, the neurotoxin tetrodotoxin is a pyrimidine derivative. It is found in a number of species including the Japanese puffer fish, the blue-ringed octopus, and the orange-bellied newt. Tetrodotoxin prevents the transmission of nerve signals and can result in paralysis and death.SDS of cas: 35139-67-4
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