Kawasaki, Fumiko; Beraldi, Dario; Hardisty, Robyn E.; McInroy, Gordon R.; van Delft, Pieter; Balasubramanian, Shankar published an article in 2017, the title of the article was Genome-wide mapping of 5-hydroxymethyluracil in the eukaryote parasite Leishmania.Application of 4433-40-3 And the article contains the following content:
Background: 5-Hydroxymethyluracil (5hmU) is a thymine base modification found in the genomes of a diverse range of organisms. To explore the functional importance of 5hmU, we develop a method for the genome-wide mapping of 5hmU-modified loci based on a chem. tagging strategy for the hydroxymethyl group. Results: We apply the method to generate genome-wide maps of 5hmU in the parasitic protozoan Leishmania sp. In this genus, another thymine modification, 5-(β-glucopyranosyl) hydroxymethyluracil (base J), plays a key role during transcription. To elucidate the relationship between 5hmU and base J, we also map base J loci by introducing a chem. tagging strategy for the glucopyranoside residue. : bserved 5hmU peaks are highly consistent among tech. replicates, confirming the robustness of the method. 5hmU is enriched in strand switch regions, telomeric regions, and intergenic regions. Over 90% of 5hmU-enriched loci overlapped with base J-enriched loci, which occurs mostly within strand switch regions. We also identify loci comprising 5hmU but not base J, which are enriched with motifs consisting of a stretch of thymine bases. Conclusions: By chem. detecting 5hmU we present a method to provide a genome-wide map of this modification, which will help address the emerging interest in the role of 5hmU. This method will also be applicable to other organisms bearing 5hmU. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Application of 4433-40-3
The Article related to genome wide mapping 5hydroxymethyluracil transcription dna sequencing leishmania, 5-formyluracil (5fu), 5-hydroxymethyluracil (5hmu), base j, genome-wide mapping, leishmania donovani, leishmania major and other aspects.Application of 4433-40-3
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia