Kropinski, Andrew M.; Turner, Dann; Nash, John H. E.; Ackermann, Hans-Wolfgang; Lingohr, Erika J.; Warren, Richard A.; Ehrlich, Kenneth C.; Ehrlich, Melanie published an article in 2018, the title of the article was The sequence of two bacteriophages with hypermodified bases reveals novel phage-host interactions.HPLC of Formula: 4433-40-3 And the article contains the following content:
Bacteriophages SP-15 and ΦW-14 are members of the Myoviridae infecting Bacillus subtilis and Delftia (formerly Pseudomonas) acidovorans, resp. What links them is that in both cases, approx. 50% of the thymine residues are replaced by hypermodified bases. The consequence of this is that the physico-chem. properties of the DNA are radically altered (melting temperature (Tm), buoyant d. and susceptibility to restriction endonucleases). Using 454 pyrosequencing technol., we sequenced the genomes of both viruses. Phage ΦW-14 possesses a 157-kb genome (56.3% GC) specifying 236 proteins, while SP-15 is larger at 222 kb (38.6 mol % G + C) and encodes 318 proteins. In both cases, the phages can be considered genomic singletons since they do not possess BLASTn homologs. While no obvious genes were identified as being responsible for the modified base in ΦW-14, SP-15 contains a cluster of genes obviously involved in carbohydrate metabolism The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).HPLC of Formula: 4433-40-3
The Article related to carbohydrate metabolism myoviridae bacillus delftia, 5-hydroxymethyluracil, 5-hydroxypentyluracil, bacillus, dna sequencing, delftia, sp-15, alpha-putrescinylthymine, bacteriophage, hypermodified bases, φw-14 and other aspects.HPLC of Formula: 4433-40-3
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia