Leng, Jiapeng’s team published research in Journal of the American Society for Mass Spectrometry in 22 | CAS: 56-05-3

Journal of the American Society for Mass Spectrometry published new progress about 56-05-3. 56-05-3 belongs to pyrimidines, auxiliary class Pyrimidine,Chloride,Amine,API, name is 2-Amino-4,6-dichloropyrimidine, and the molecular formula is C4H3Cl2N3, Product Details of C4H3Cl2N3.

Leng, Jiapeng published the artcileIntegration of high accuracy N-terminus identification in peptide sequencing and comparative protein analysis via isothiocyanate-based isotope labeling reagent with ESI ion-trap TOF MS, Product Details of C4H3Cl2N3, the publication is Journal of the American Society for Mass Spectrometry (2011), 22(7), 1204-1213, database is CAplus and MEDLINE.

A multifunctional isothiocyanate-based isotope labeling reagent, [d0]-/[d6]-4,6-dimethoxy pyrimidine-2-isothiocyanate (DMPITC), has been developed for accurate N-terminus identification in peptide sequencing and comparative protein anal. by ESI Ion-trap TOF mass spectrometry. In contrast with the conventional labeling reagent Ph isothiocyanate (PITC), DMPITC showed more desirable properties such as rapid labeling, sensitivity enhancement, and facilitating peptide sequencing. More significantly, DMPITC-based labeling strategy possessed the capacity of higher reliable N-terminus identification owning to the high-yield b1 ion combined with the isotope validation of 6 Da. Meanwhile, it also showed potential in differentiating isomeric residues of leucine and isoleucine at N-terminus on the basis of the relative abundance ratios between the fragment ions of their resp. b1 ions. The strategy not only allows accurate interpretation for peptide but also ensures rapid and sensitive comparative anal. for protein by direct MS anal. Using trypsin-digested bovine serum albumin (BSA), both peptide N-terminus identification and quant. anal. were accomplished with high accuracy, efficiency, and reproducibility. The application of DMPITC-based labeling strategy is expected to serve as a promising tool for proteome research.

Journal of the American Society for Mass Spectrometry published new progress about 56-05-3. 56-05-3 belongs to pyrimidines, auxiliary class Pyrimidine,Chloride,Amine,API, name is 2-Amino-4,6-dichloropyrimidine, and the molecular formula is C4H3Cl2N3, Product Details of C4H3Cl2N3.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia