Human cytomegalovirus-induced DNA polymerase and its interaction with the triphosphates of 1-(2′-deoxy-2′-fluoro-β-D-arabinofuranosyl)-5-methyluracil, -5-iodocytosine, and -5-methylcytosine was written by Mar, Eng Chun; Chiou, Jwo Farn; Cheng, Yung Chi; Huang, Eng Shang. And the article was included in Journal of Virology on December 31,1985.Computed Properties of C10H13FN2O5 The following contents are mentioned in the article:
Human cytomegalovirus-induced DNA polymerase [9012-90-2] and cellular DNA polymerase α were purified by successive chromatog. on DEAE-cellulose, phosphocellulose, heparin agarose, and single-stranded DNA agarose columns. The purified virus-induced DNA polymerase was resolved to 2 polypeptides corresponding to mol. weights of 140,000 and 58,000, as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Virus-induced DNA polymerase and cellular α polymerase were examined for their sensitivities to the triphosphates of 1-(2′-deoxy-2′-fluoro-β-D-arabinofuranosyl)-5-methyluracil (FMAUTP) [69256-17-3], -5-iodocytosine (FIACTP) [69123-90-6], and -5-methylcytosine (FMACTP) [78636-53-0]. The inhibitive effects of these triphosphates on the DNA polymerases were competitive with regard to the natural substrates; thus FMAUTP completes with dTTP [365-08-2], and FIACTP and FMACTP complete with dCTP [2056-98-6]. The inhibition constants (Ki) for FMAUTP, FIACTP, and FMACTP of virus-induced DNA polymerase are 0.06, 0.30, and 0.47 μM, resp. Cellular DNA polymerase α is much less sensitive to these inhibitors, and its Ki values for FMAUTP, FIACTP, and FMACTP are 0.45, 3.10, and 2.90 μM, resp. In addition, human cytomegalovirus-induced DNA polymerase, but not cellular DNA polymerase α, can utilize these analog triphosphates as alternate substrates for their corresponding natural deoxyribonucleoside triphosphates in in vitro DNA synthesis. This study involved multiple reactions and reactants, such as 1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3Computed Properties of C10H13FN2O5).
1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (cas: 69256-17-3) belongs to pyrimidine derivatives. Pyrimidine is an aromatic heterocyclic organic compound similar to pyridine. As nucleotides in DNA and RNA, pyrimidine nucleotide derivatives have a wide range of biological applications. For example, pyrimidine derivatives are useful in DNA repair studies involving cancer and epigenetics.Computed Properties of C10H13FN2O5
69256-17-3;1-((2R,3S,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione;The future of 69256-17-3;New trend of C10H13FN2O5;function of 69256-17-3