Si, Xingyu published the artcileDetecting Low-Abundance Molecules at Single-Cell Level by Repeated Ion Accumulation in Ion Trap Mass Spectrometer, Name: 5-Methylcytosinehydrochloride, the main research area is low abundance mol detection cell ion trap mass spectrometry.
Low-abundance metabolites or proteins in single-cell samples are usually undetectable by mass spectrometry (MS) due to the limited amount of substances in single cells. This limitation inspired the authors to further enhance the sensitivity of com. mass spectrometers. Herein, the authors developed a technique named repeated ion accumulation by ion trap MS, which is capable of enhancing the sensitivity by selectively and repeatedly accumulating ions in a linear ion trap for up to 25 cycles. The increase in MS sensitivity was pos. correlated with the number of repeated cycles. When ions were repeatedly accumulated for 25 cycles, the sensitivity of ATP detection was increased by 22-fold within 1.8 s. The authors’ technique could stably detect low-abundance ions, especially MSn ions, at the single-cell level, such as 5-methylcytosine hydrolyzed from sample equivalent to ∼0.2 MCF7 cell. The strategy presented in this study offers the possibility to aid single-cell anal. by enhancing MS detection sensitivity.
Analytical Chemistry (Washington, DC, United States) published new progress about Electrospray ionization mass spectrometry (nanospray ionization, ion trap). 58366-64-6 belongs to class pyrimidines, name is 5-Methylcytosinehydrochloride, and the molecular formula is C5H8ClN3O, Name: 5-Methylcytosinehydrochloride.
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia