Reference of 1981-58-4, As an important bridge between the micro and macro material world, chemistry is one of the main methods and means for humans to understand and transform the material world. 1981-58-4, Name is Sulfamethazine sodium, SMILES is CC1=CC(C)=NC([N-]S(=O)(C2=CC=C(N)C=C2)=O)=N1.[Na+], belongs to pyrimidines compound. In a article, author is Suprun, Elena, V, introduce new discover of the category.
Deoxyuridine triphosphates modified with tyrosine or tryptophan aromatic groups for direct electrochemical detection of double-stranded DNA
Deoxyuridine triphosphates (dUTP) modified with tyrosine or tryptophan aromatic groups attached through CH=CH-CH2-NH-C(O)-(CH2)(n)- linker at the C5 position of the pyrimidine ring and the corresponding products of polymerase chain reaction (PCR) were studied by cyclic and square wave voltammetry on carbon screen printed electrodes. A strong effect of the additional functional groups on the electrochemical activity of nucleotides was revealed. In particular, 5-aminoallyl-2′-deoxyuridine-5′-triphosphates modified with indole acetic, indole-3-propionic, indole-4-butyric, or 4-hydroxyphenylacetic acids demonstrated novel well-defined oxidation peaks at 0.5-0.7 V, similar to tryptophan or tyrosine amino acids, respectively. The oxidation potential maxima for dUTP derivatives under study were about 0.2-0.3 V less positive than the oxidation potential of dGTP (the most easily oxidizable nucleotide). Moreover, dUTP derivatives modified with tyrosine or tryptophan aromatic groups were incorporated by PCR into dsDNA fragments (amplicons) instead of dITP. The PCR-generated dsDNA fragments with modified nucleotides were detected through the oxidation of their electroactive ‘labels’ at micromolar concentrations, while no oxidation peaks were observed for unmodified amplicons under the same conditions. The tyrosine and tryptophan aromatic groups well complement the existing palette of electroactive tags for direct detection of nucleic acids. Compared to other electroactive ‘labels’, the main advantage of the developed oxidizable moieties is a good compatibility with polymerase enzymes including those used in PCR. In addition, the demonstrated signal recording procedure for modified amplicons on disposable carbon electrodes renders itself suitable for in situ analysis of biological samples for biochemical and medical applications. (C) 2020 Elsevier Ltd. All rights reserved.
Reference of 1981-58-4, Each elementary reaction can be described in terms of its molecularity, the number of molecules that collide in that step. The slowest step in a reaction mechanism is the rate-determining step.you can also check out more blogs about 1981-58-4.
Reference:
Pyrimidine | C4H4N2 – PubChem,
,Pyrimidine – Wikipedia