Synthetic Route of 151266-23-8, Children learn through play, and they learn more than adults might expect. Science experiments are a great way to spark their curiosity, 151266-23-8, Name is 3-Iodo-1H-pyrazolo[3,4-d]pyrimidin-4-amine, SMILES is NC1=C2C(NN=C2I)=NC=N1, belongs to pyrimidines compound. In a article, author is Pfeiffer, Martin, introduce new discover of the category.
Reverse C-glycosidase reaction provides C-nucleotide building blocks of xenobiotic nucleic acids
C-Analogues of the canonical N-nucleosides have considerable importance in medicinal chemistry and are promising building blocks of xenobiotic nucleic acids (XNA) in synthetic biology. Although well established for synthesis of N-nucleosides, biocatalytic methods are lacking in C-nucleoside synthetic chemistry. Here, we identify pseudouridine monophosphate C-glycosidase for selective 5-beta -C-glycosylation of uracil and derivatives thereof from pentose 5-phosphate (d-ribose, 2-deoxy-d-ribose, d-arabinose, d-xylose) substrates. Substrate requirements of the enzymatic reaction are consistent with a Mannich-like addition between the pyrimidine nucleobase and the iminium intermediate of enzyme (Lys166) and open-chain pentose 5-phosphate. beta -Elimination of the lysine and stereoselective ring closure give the product. We demonstrate phosphorylation-glycosylation cascade reactions for efficient, one-pot synthesis of C-nucleoside phosphates (yield: 33 – 94%) from unprotected sugar and nucleobase. We show incorporation of the enzymatically synthesized C-nucleotide triphosphates into nucleic acids by RNA polymerase. Collectively, these findings implement biocatalytic methodology for C-nucleotide synthesis which can facilitate XNA engineering for synthetic biology applications.C-nucleosides are analogues of the canonical N-nucleosides and, despite their synthetic value, biocatalysis has not targeted them yet. Here, the authors report a pseudouridine monophosphate C-glycosidase enzyme for selective 5-beta -C-glycosylation of uracil and its derivatives from pentose 5- phosphate substrates.
Synthetic Route of 151266-23-8, Each elementary reaction can be described in terms of its molecularity, the number of molecules that collide in that step. The slowest step in a reaction mechanism is the rate-determining step.you can also check out more blogs about 151266-23-8.
Reference:
Pyrimidine | C4H4N2 – PubChem,
,Pyrimidine – Wikipedia