Microbiological inhibition-based method for screening and identifying of antibiotic residues in milk, chicken egg and honey was written by Wu, Qin;Shabbir, Muhammad Abu Bakr;Peng, Dapeng;Yuan, Zonghui;Wang, Yulian. And the article was included in Food Chemistry in 2021.Electric Literature of C11H12N4O3S This article mentions the following:
This present study was designed to develop a novel microbiol. inhibition-based method for the rapid screening and identification of antibiotic residues in milk, chicken egg and honey. Geobacillus stearothermophilus C953 was used as test bacterium in the detection system of this study. The optimization of nutrients and other supplements were performed to promote the growth of test bacterium and thus shorten the detection time. Furthermore, the synergetic agents were added to improve the sensitivity of test bacterium to more antibiotics. Addnl., confirmatory solutions such as β-lactamase, p-aminobenzoic acid, MgSO4 and cysteine were added to classify and identify different kinds of antibiotics. We observed that the LOD of this detection system was at or close to maximum residue limits established by EU for β-lactams, aminoglycosides, tetracyclines, sulfonamides, macrolides and quinolones in milk. The LOD of different kinds of antibiotics in chicken egg was less than or similar to the MRL and the LOD of Premitest (except sulfonamides). For honey, there are no MRL, the LOD was less than or similar to the recommended concentration and the LOD of Premitest. Noteworthy, the detection system also can identify these six kinds of antibiotics in milk, chicken egg and honey, and there were satisfactory results of specificity experiments and confirmation experiments by LC-MS/MS. Accordingly, the present study provides a reliable preliminary characterization of antibiotic residues in animal foods and improves the detection efficiency for the following chem. confirmation experiments by HPLC, LC-MS/MS, immunol. and receptor-based tests. In the experiment, the researchers used many compounds, for example, 4-Amino-N-(6-methoxypyrimidin-4-yl)benzenesulfonamide (cas: 1220-83-3Electric Literature of C11H12N4O3S).
4-Amino-N-(6-methoxypyrimidin-4-yl)benzenesulfonamide (cas: 1220-83-3) belongs to pyrimidine derivatives. The pyrimidine nitrogenous bases are derived from the organic compound pyrimidine through the addition of various functional groups. A Cu-catalyzed and 4-HO-TEMPO-mediated [3 + 3] annulation of commercially available amidines with saturated ketones enables an efficient and facile synthesis of structurally important pyrimidines via a cascade reaction of oxidative dehydrogenation/annulation/oxidative aromatization.Electric Literature of C11H12N4O3S
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia