Inhibition of H3K27me3 demethylases attenuates asthma by reversing the shift in airway smooth muscle phenotype was written by Yu, Qijun;Yu, Xiaowei;Zhao, Wenxue;Zhu, Manni;Wang, Zhengxia;Zhang, Jiaxiang;Huang, Mao;Zeng, Xiaoning. And the article was included in Clinical & Experimental Allergy in 2018.Product Details of 1373423-53-0 The following contents are mentioned in the article:
The shift in airway smooth muscle cells (ASMCs) phenotype between proliferation and contraction during asthma has been reported recently, highlighting a role of ASMCs plasticity in the pathophysiol. of asthma. As an event involved in epigenetic post-translational modification, histone H3 lysine27 (H3K27) demethylation has attracted significant attention with respect to the epigenetic changes in diverse cells; however, little is known about its contribution to the switching of ASMCs phenotype in asthma. To investigate the role of trimethylated H3K27 (H3k27me3) demethylation in ASM remodeling as well as the underling mechanism. Mice were exposed five times a week to house dust mite (HDM) extract for 5 wk. Lung function was measured following the final HDM challenge. Airway inflammation and remodeling were then assessed in lungs of individual mice. Human ASMCs were purchased from Sciencell Research Laboratories Proliferation, synthesis, migration and contraction of ASMCs were analyzed, resp. We observed demethylation at H3k27me3 sites in lungs harvested from mice exposed to HDM extract Administration of a selective inhibitor of H3K27 demethylase (GSK-J4) could ameliorate the classical hallmarks of asthma, such as airway hyperresponsiveness, airway inflammation and remodeling. We established a proliferative as well as a contractive model of human ASMCs to explore the impacts of H3K27 demethylase inhibition on ASMCs phenotype. Our results indicated that GSK-J4 decreased ASMCs proliferation and migration elicited by PDGF through the Akt/JNK signalling; GSK-J4 also prevented the upregulation of contractile proteins in ASMCs induced by TGF-β through the Smad3 pathway. Inhibition of H3K27me3 demethylation alleviated the development of asthmatic airway disease in vivo and modulated ASMCs phenotype in vitro. Collectively, our findings highlight a role of H3K27me3 demethylation in exptl. asthma and ASMCs phenotype switch. This study involved multiple reactions and reactants, such as Ethyl 3-((6-(4,5-dihydro-1H-benzo[d]azepin-3(2H)-yl)-2-(pyridin-2-yl)pyrimidin-4-yl)amino)propanoate (cas: 1373423-53-0Product Details of 1373423-53-0).
Ethyl 3-((6-(4,5-dihydro-1H-benzo[d]azepin-3(2H)-yl)-2-(pyridin-2-yl)pyrimidin-4-yl)amino)propanoate (cas: 1373423-53-0) belongs to pyrimidine derivatives. Pyrimidine is an aromatic heterocyclic organic compound similar to pyridine. Pyrimidine derivatives also play an important role in drug development, either in concert with other compounds or on their own.Product Details of 1373423-53-0
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Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia