Suspect screening and untargeted analysis of veterinary drugs in food by LC-HRMS: Application of background exclusion-dependent acquisition for retrospective analysis of unknown xenobiotics was written by Zhu, Chunyan;Lai, Guoyin;Jin, Ying;Xu, Dunming;Chen, Jiayun;Jiang, Xiaojuan;Wang, Suping;Liu, Guoqiang;Xu, Niusheng;Shen, Rong;Wang, Luxiao;Zhu, Mingshe;Wu, Caisheng. And the article was included in Journal of Pharmaceutical and Biomedical Analysis in 2022.Recommanded Product: 4-Amino-N-(6-methoxypyrimidin-4-yl)benzenesulfonamide This article mentions the following:
The presence of veterinary drug and pesticide residues in food products pose considerable threats to human health. Monitoring of these residues in food is mainly carried out using targeted anal. by triple quadrupole mass spectrometry. However, these methods are not suitable for suspect screening and untargeted anal. of unknowns. The main objectives of this study were to develop a new high-resolution mass spectrometry (HRMS)-based anal. strategy for retrospective anal. of suspect and unknown xenobiotics and to evaluate its performance in the tentative identification of 48 veterinary drugs as “unknowns” spiked in a pork sample. In the anal., a newly developed background exclusion data-dependent acquisition (BE-DDA) technique was employed to trigger the product ion (MS/MS) spectral acquisition of the “unknowns”, and an inhouse precise-and-thorough background-subtraction (PATBS) technique was applied to detect these “unknowns”. Showed that untargeted data mining of the acquired LC-MS dataset by PATBS was able to find all the 48 veterinary drugs and 46 of them were triggered by BE-DDA to generate accurate MS/MS spectra. The dataset of recorded accurate full-scan mass and MS/MS spectra of all the xenobiotics of the test pork sample is defined as the xenobiotics profile. Searching the xenobiotic profile of the test pork sample using mass spectral data of selected veterinary drugs (as suspects) from the mzCloud spectral library led to the correct hits. Searching against the mzCloud spectral library using the mass spectral data of selected individual veterinary drugs (as unknowns) from the xenobiotics profile tentatively confirmed their identities. In contrast, anal. of the same sample using ion intensity-data dependent acquisition only recorded the MS/MS spectra for 34 veterinary drugs. In addition, a data independent acquisition method enabled the acquisition of the fragment spectra for 44 veterinary drugs, but their spectral data displayed only one or a few true product ions of individual analytes of interest along with many fragments from coeluted biol. components and background noises. This study demonstrates that this anal. strategy has a potential to become a practical tool for the retrospective suspect screening and untargeted anal. of unknown xenobiotics in a biol. sample such as veterinary drugs and pesticides in food products. In the experiment, the researchers used many compounds, for example, 4-Amino-N-(6-methoxypyrimidin-4-yl)benzenesulfonamide (cas: 1220-83-3Recommanded Product: 4-Amino-N-(6-methoxypyrimidin-4-yl)benzenesulfonamide).
4-Amino-N-(6-methoxypyrimidin-4-yl)benzenesulfonamide (cas: 1220-83-3) belongs to pyrimidine derivatives. Pyrimidine also found in many synthetic compounds such as barbiturates and the HIV drug, zidovudine. For example, the neurotoxin tetrodotoxin is a pyrimidine derivative. It is found in a number of species including the Japanese puffer fish, the blue-ringed octopus, and the orange-bellied newt. Tetrodotoxin prevents the transmission of nerve signals and can result in paralysis and death.Recommanded Product: 4-Amino-N-(6-methoxypyrimidin-4-yl)benzenesulfonamide
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia